土壤中分泌嗜铁素细菌的筛选及影响其分泌因素的研究

园艺学报 ›› 2007, Vol. 34 ›› Issue (4): 1023-1028.

土壤中分泌嗜铁素细菌的筛选及影响其分泌因素的研究

梁建根; 竺利红; 吴吉安; 李孝辉; 桑金隆; 姚杭丽; 施跃峰^*

(浙江省农业科学院植物保护与微生物研究所, 杭州310021)

收稿日期:2007-01-28 修回日期:2007-04-22 出版日期:2007-08-25 发布日期:2007-08-25

Studies on Screening of Siderophore Producing Bacteria in the Soil and Factors Influencing Its Secretion

LIANG Jian-gen; ZHU Li-hong; WU Ji-an; LI Xiao-hui; SANG Jin-long; YAO Hang-li; SHI Yue-feng^*

( Institute of Plant Protection and Microbiology, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021,China)

Received:2007-01-28 Revised:2007-04-22 Online:2007-08-25 Published:2007-08-25

摘要/Abstract

摘要： 对土壤中分泌嗜铁素的细菌进行了筛选并初步研究了影响其分泌的因素。结果表明,采用经典的铬奥醇CAS(chrome azurol S)选择性培养基法,并结合培养液在紫外灯下有无黄绿色的荧光产生法,这两种筛选方法的结果是相吻合的。从嗜铁素产生光吸收峰的数量与强度可以断定,不同的pH值会产生不同分子结构的嗜铁素,pH8.3最适合试验菌HZX-25产生嗜铁素。SM是最适合富产嗜铁素的培养基。C/N比10∶1最适合菌株HZX-25分泌嗜铁素。不同外源氨基酸对嗜铁素产生有着不同作用。嗜铁素上清液都显著地抑制了5种植物病原菌的菌丝生长(P=0.05)。

关键词: 细菌, 嗜铁素, 筛选, 分泌因素

Abstract: Screening of siderophore producing bacteria in the soil and factors influencing its secretion were studied. Results of two methods using selected-medium CAS(chrome azurol S) and fluorescence observation under violet light to screen siderophore producing bacteria were consistent. By the virtue of quantity and intensity of siderophore absorbance apex, it is concluded that different molecular structures of siderophore appeared under different pH values. In addition, pH 8.3, SM medium and C∶N ratio of 10∶1 represented the optimum to produce siderophore, respectively. Different exogenous amino acids had different effects on production of siderophore. Supernatant of siderophore had a significantly inhibitory effect on five different phytopathogenic moulds growth.

梁建根;竺利红;吴吉安;李孝辉;桑金隆;姚杭丽;施跃峰. 土壤中分泌嗜铁素细菌的筛选及影响其分泌因素的研究[J]. 园艺学报, 2007, 34(4): 1023-1028.

LIANG Jian-gen;ZHU Li-hong;WU Ji-an;LI Xiao-hui;SANG Jin-long;YAO Hang-li;SHI Yue-feng. Studies on Screening of Siderophore Producing Bacteria in the Soil and Factors Influencing Its Secretion[J]. ACTA HORTICULTURAE SINICA, 2007, 34(4): 1023-1028.

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