关键词: 甘蓝, 远缘杂交, 胚挽救, 鉴定

Abstract: In order to transfer the Ogura CMS restorer gene of Brassica napus to the Ogura CMS lines of Brassica oleracea，6 Ogura CMS lines of cabbage and the B. napus which contained the restorer gene were used as the female and male parents respectively to obtain the F1 generation through embryo rescue culture and the artificial hybridization technology，and their authenticity was also identified. Investigation was carried out on the factors which could affect ovule seedling formation，including sampling time，medium composition and cross combination. The results showed that the ovular seedling formation efficiency was the highest while using ovules 16 d after pollination，altogether the seedling rate was 4.56%. The medium of MS + GA 0.1 mg ? L-1 + NAA 0.1 mg ? L-1 + 0.5% casein hydrolyzate + 0.5% activated carbon，had the best seedling growing-up with the seedling rate as high as 6.24%. The cross combination of M09CMS × RFO-46 generated the most F1 generation seedlings with the 5.96% seedling rate. Sixty five out of 67 F1 generation seedlings were identified as the true hybrids through flow cytometry，SSR molecular markers and morphological identification，accounting for 97%.

Key words: cabbage, distant hybridization, embryo rescue, identification