关键词: 甜瓜, 果腐病, 变红镰刀菌, 鉴定, 生物学特性

Abstract: A novel disease of suspected Fusarium fruit rot was observed on muskmelon in Taigu County，Shanxi Province. The isolate was obtained from diseased sample by conventional tissue isolation method. The pathogenicity was confirmed by Koch’s postulates. The pathogen was identified as Fusarium incarnatum based on morphological characteristics and the sequence analysis of rDNA-ITS，EF-1α，histone 3 and mtSSU. The results of biological characteristics showed that PSA medium was most conducive to mycelium growth and sporulation；the ideal temperature conditions for mycelium growth and sporulation were in the range of 20–35 ℃ and 20–25 ℃，respectively；the suitable carbon sources for mycelium growth were CMC-Na，starch and xylose，the optimal carbon source for sporulation was lactose. The pathogen could grow fastly on the medium with kinds of nitrogen sources，the optimal nitrogen source for sporulation was yeast powder. The optimal pH for mycelium growth was 8. The full illumination and full darkness was suitable for mycelium growth. The sporulation under pH and light conditions had not significant difference. The mycelial lethal temperature was at 61 ℃ for 10 min，and the spore lethal temperature was at 56 ℃ for 10 min.

Key words: muskmelon, fruit rot, Fusarium incarnatum, identification, biological characteristics