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园艺学报 ›› 2007, Vol. 34 ›› Issue (4): 1015-1018.

• 研究简报 • 上一篇    下一篇

不同抗性番茄品系接种晚疫病菌后超微结构的变化

李从顺; 金兆辉; 朱海山*
  

  1. (云南农业大学园林园艺学院园艺系, 昆明650201)
  • 收稿日期:2007-02-05 修回日期:2007-04-28 出版日期:2007-08-25 发布日期:2007-08-25

Changes of Cell Ultra Structure in Different Resistant Lines of Tomato afterPhytophthora infestans Inoculation

LI Cong-shun; JIN Zhao-hui; ZHU Hai-shan*
  

  1. (Department of Horticulture, College of Landscape and Horticulture, Yunnan Agricultural University, Kunming 650201, China)
  • Received:2007-02-05 Revised:2007-04-28 Online:2007-08-25 Published:2007-08-25

摘要: 对不同抗性的番茄品系(自交系5号、CLN2037E及杂交一代) 人工接种晚疫病菌后, 观察
叶片超微结构变化。结果表明: (1) 接菌前, 自交系5号的线粒体比CLN2037E和F1多。(2) 接菌42 h,
3个品系都出现质壁分离; 自交系5号和F1的线粒体增加, 同时出现过氧化物酶体; 自交系5号叶绿体被
破坏。(3) 接菌54 h, CLN2037E线粒体增加, 出现过氧化物酶体; F1有部分叶绿体破坏。(4) 接菌72 h,
3个品系都出现细胞壁折叠, 自交系5号的叶绿体只存在零散的基质、基粒片层, 少数线粒体膜破坏和空
泡化; F1的多数叶绿体还存在基本形状; CLN2037E只有少数的叶绿体膜破坏。结论: 接种病菌后, 抗性
品系(CLN2037E) 比感病品系(自交系5号) 叶绿体被破坏程度轻, 线粒体增加和过氧化物酶体出现时
间晚, 细胞壁折叠很明显。

关键词: 番茄, 叶片, 晚疫病菌, 超微结构

Abstract: Three different resistant tomato lines ( Inbred line 5, CLN2037E and their cross F1 ) were stud
ied in incubator after inoculating Phytophthora infestans. We took leaves at different time to observe the deterio
ration of leaves ultra structure. The result showed that: first, before inoculating Phytophthora infestans, Inbred
line 5 hasmore mitochondria than F1 and CLN2037E. Second, inoculated 42 h, three lines appeared p lasmoly
sis. Both Inbred line 5 and F1 appeared peroxisome and the mitochondria quantity increased, and chlorop last of
Inbred line 5 was also damaged. Third, inoculated 54 h, CLN2037E appeared peroxisome and mitochondria
quantity increased; also a part of chlorop last of F1 was destroyed. Fourth, inoculated 72 h, the cell wall in all
three lines became creased. Much of the chlorop lastmembrane of inbred line 5 was decomposed. There was only
scattered thylakoid and grana lamella, a few mitochondria cavitations and membrane was damaged. There was
basic chlorop last structure in F1 and little chlorop last membrane of CLN2037E was damaged. Conclusion: we
compared resistant line (CLN2037E) with sensitive line ( Inbred line 5) after inoculating Phytophthora infes
tans,
and found that the structure of resistant line chlorop lastwas least damaged. Peroxisome appeared later and
increased quantities ofmitochondria also appeared at later stage. Creasing in the cell wall became obvious.

Key words: Tomato, Leaf, Phytophthora infestans, Ultra structure