关键词: 茄子, UV-B, SmUVR8, SmCOP1, SmHY5, SmCHS

Abstract:

Ultraviolet-B（UV-B）receptor gene UVR8 was cloned from eggplant（Solanum melongena L.）using homology-based cloning method，named SmUVR8. Sequence alignment shows SmUVR8 is similar to UVR8 in other Solanaceae plant and Arabidopsis thaliana，indicating SmUVR8 may have the function of AtUVR8. To further study the function of the SmUVR8，SmUVR8 was transformated in the Arabidopsis uvr8 mutant. The result showed SmUVR8 restored the phenotype of hypocotyl elongation，the flowering time and anthocyanin accumulation of Arabidopsis uvr8 mutant，which indicated eggplant SmUVR8 gene have the similar function to the Arabidopsis AtUVR8 gene. Real-time fluorescent quantitation and yeast two hybrid result reveals UV-B signal can influence photomorphogenesis of eggplant by the interaction of SmUVR8 and SmCOP1 activating SmHY5 and SmCHS .

Key words: Solanum melongena, UV-B, SmUVR8, SmCOP1, SmHY5, SmCHS