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园艺学报 ›› 2019, Vol. 46 ›› Issue (2): 252-264.doi: 10.16420/j.issn.0513-353x.2018-0396

• 研究论文 • 上一篇    下一篇

番木瓜ERF家族与果实成熟相关成员的分析

陈永萍1,高 峰2,申艳红3,赵湾湾4,陈桂信1,*,王 平4,*   

  1. 1福建农林大学园艺学院,福州 350002;2福建农林大学生命科学学院,福州 350002;3河北科技师范学院园艺科技学院,河北秦皇岛 066004;4福建农林大学园艺学院园艺植物遗传育种研究所,福州 350002
  • 出版日期:2019-02-25 发布日期:2019-02-25
  • 基金资助:

    福建农林大学科技发展基金项目(KFA17355A)

The Analysis of ERFs Related to Fruit Ripening in Papaya

CHEN Yongping1,GAO Feng2,SHEN Yanhong3,ZHAO Wanwan4,CHEN Guixin1,*,and WANG Ping4,*   

  1. 1College of Horticulture,Fujian Agriculture and Forestry University,Fuzhou 350002,China;2College of Life Science,Fujian Agriculture and Forestry University,Fuzhou 350002,China;3College of Horticulture Science and Technology,Hebei Normal University of Science and Technology,Qinhuangdao,Hebei 066004,China;4Institute of Genetics and Breeding in Horticultural Plants,College of Horticulture,Fujian Agriculture and Forestry University,Fuzhou 350002,China
  • Online:2019-02-25 Published:2019-02-25

摘要:

通过比较分析番木瓜ERF家族成员的系统进化关系、基本理化性质、保守基序、启动子顺式元件,结合分析番木瓜在乙烯利和乙烯抑制剂1–甲基环丙烯(1-MCP)处理下以及不同成熟期果肉的RNA-seq数据,筛选出22个ERF家族成员进行表达差异分析,最终比较得出两个处理数据中显著差异表达基因有18个,推测这些基因可能在番木瓜果实成熟过程中起重要作用。

关键词: 番木瓜, ERF, 果实, 成熟, 转录因子

Abstract:

Through the comparative analysis of phylogenetic relationships,basic physical and chemical properties,conservative motifs,the promoter cis-elements of CpERFs and combining with papaya RNA-seq data,which conclude different treatments [ethylene and ethylene inhibitor 1-methyl propylene(1-MCP)treatments] and different ripening stages in pulp,this research selected 22 CpERFs members for expression analysis. Through RNA-seq 18 members of ERF family were found,which suggests that these genes may play important role in response to fruit ripening in papaya.

Key words: Carica papaya, ERF;fruit, ripening, transcription factor

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