关键词: 葡萄, 叶片, 衰老, 补充光照, 红光, 蓝光, 光合特性, 叶绿体超微结构

Abstract:

The experiment was carried out in 2013 at the fruit garden of the Chinese Academy of Agricultural Sciences in Liaoning，which was equipped with a core grape technology demonstration garden.‘Italy’grape cultivars were grafted on Beta as test materials. And their row spacing was 0.7 m，line spacing was 2 m. The lean dragon trunk matches the V shape leaf curtain，and used the integrated management of water and fertilizer，other management was the same as the routine. Since the beginning of August 1st to the defoliation day before dark for half an hour to fill 24：00，use red light and blue light to deal with the grape cultivars，with no-fill-light grapes as contrast，studying morphological characteristics，chlorophyll content，net photosynthetic rate，Fv/Fm value，soluble proteins content and chloroplast ultrastructure of grape leaves in aging process，to provide theoretical basis for the research and development of leaf anti-aging technology. The research result showed that compared with the control group，red light treatment increased the chlorophyll content，net photosynthetic rate，Fv/Fm value and soluble proteins content of leaf，the chloroplast grana were arranged in order，the disintegration of chloroplast ultrastructure was obviously slowed down，leaf senescence was delayed significantly. Blue light treatment significantly reduced the leaf chlorophyll content，net photosynthetic rate，soluble proteins content and Fv/Fm value，the ultrastructure of chloroplasts was destroyed，and the aging process of grapes was accelerated. But in the later stage of leaf senescence，the chlorophyll content of leaf，the net photosynthetic rate，soluble proteins content and Fv/Fm value of the blue light treatment was higher than that of the control. Moreover，the grana lamellae of leaf chloroplasts are arranged in order，leaf senescence was delayed in some degree. The content of soluble proteins in leaf of blue light treatment was significantly higher than that of red light treatment and control group. Summing up the above，the red light as supplement light had the best effect，the leaf senescence process was delayed effectively，and the physiological function of leaves was prolonged.

Key words: grape, leaf, senescence, fill light, red light, blue light, photosynthetic property, chloroplast ultrastructure