关键词: 杨梅, 基因组, SSR

Abstract:

The distribution of simple sequence repeat（SSR）was studied in 1 431 scaffolds of Chinese bayberry（Myrica rubar Sieb. et Zucc.）genome. There were 43 842 SSR loci identified totally. And 194 kinds of repeating motifs that distributed in 623 scaffolds were discovered. The number and frequency of dinucleotide SSR loci were 36 383 and 82.99%. The frequency of AG/CT in dinucleotide was 55.70%，and in all SSR loci was 46.23%. It was indicated that AG/CT was the most common repeat motifs in Chinese bayberry genome. The number of trinucleotide SSR loci was 6 115，accounting for 13.95%. The number of tetranucleotide，pentanucleotide and hexanucleotide were 827，245 and 272 with the frequency 1.89%，0.56% and 0.62%，respectively. Meanwhile，59 pairs of Genomic SSR markers（gSSRs）were developed. After polymorphism analysis，26 gSSRs were highly polymorphic markers and 33 gSSRs were moderately polymorphic markers. In this study，the clustering analysis among the advanced selection Zaoxian 856 and the other 13 main cultivars were identified by all 59 gSSRs. According to the result，14 Chinese bayberry materials were clustered into two groups at the similarity coefficient of 0.56. It was illustrated that Zaoxian 856 was different from the other 13 varieties at the DNA level，and the similarity coefficient between Zaoxian 856 and control‘Zaose’was 0.86.

Key words: Chinese bayberry, Myrica rubar, genome, SSR