关键词: 葡萄, 组培, 茉莉酸, 不定根, 内源激素, 叶绿素

Abstract: Jasmonic acid is not only involved in plant stress resistance，but also can regulate fruit growth and development. However，little research has been done on the regulation of grape root development. In this study，we used three concentrations of MeJA to treat grapevine tissue culture，and analyzed the physiological and gene expression levels. The results showed that MeJA could inhibit adventitious roots development and elongation，increased abscisic acid（ABA）content in leaf，root，stem tissues，but decreased the contents of gibberelin（GA），auxin （IAA）and cytokinin（ZR），and also affected the metabolism of chlorophyll in leaves that led to leaf chlorosis. Moreover，jasmonic acid could induce the expression of jasmonic acid synthesis genes to further increase the content of endogenous jasmonic acid，and induce the expression of ABA synthesis genes and signal pathway. However，it could inhibit the expression of auxin，gibberellin，cytokinin，and brassinolide synthesis genes，especially，the expression of the adventitious root development and elongation-related genes VvXET，VvEXPB2，VvEXPA7 and VvWUS was inhibited，thereby inhibiting the growth of new roots，leading to slow plant development. Therefore，MeJA can affect the synthesis metabolism of other hormones to regulate their endogenous content in plants，leading to imbalance of endogenous hormones which affect plant development. MeJA plays a negative regulatory role in plant adventitious root development.

Key words: grape, tissue culture, jasmonic acid；adventitious root, hormone；chlorophyll