关键词: 丹参, 细胞分裂素调控因子, 生物信息学

Abstract:

Cytokinin plays an important role in the growth and development of plants，which responds to external environmental stimuli through the two-component system in plant. The response regulators （RRs）are critical members in the two-component system. Through genome-wide identification，15 putative SmRR genes were identified from the genome of Salvia miltiorrhiza. Based on conserved domains and phylogenetic analysis，15 SmRR genes were divided into two subfamilies，type-A（SmRR1–SmRR7）and type-B（SmRR8–SmRR15）. Sequence analysis of the SmRR gene family in S. miltiorrhiza，including gene structure，sequence features，cis-acting element，conserved motifs，and expression in different organs，were carried out. The results showed that the REC domain contains conserved DDK amino-acid residues in type-A SmRR，type-B SmRR has conserved Myb-like domain. The exon and intron number of SmRR also has relative conservatism. The analysis of cis-regulatory elements revealed that there are many stress and growth regulating substance response elements in SmRR. Differential transcript abundance was detected for SmRRs in different organs and tissues.

Key words: Salvia miltiorrhiza, cytokinin response regulator, bioinformatics