关键词: 黄瓜, 渐渗系, 蔓枯病, 遗传图谱, 混池测序, 抗病基因

Abstract: In this study，a linkage map containing 137 pairs of SSR markers and 6 pairs of InDel markers was constructed using recombinant inbred lines F2︰6 derived from a cross between parental lines‘IL77’（resistant line from the cross of C. hystrix × C. sativus）and‘8419’（susceptible line）. Genetic analysis indicated that the resistance to gummy stem blight（GSB）in‘IL77’was quantitative. Both mapping and BSA-seq analysis were used to detect quantitative trait loci（QTLs）conferring GSB resistance in cucumber leaves. The results showed that the GSB resistance genes were located in the 24.6–27.1 Mb region of chromosome 1. There were 13 candidate genes with exonic nonsynonymous SNP mutation predicted as GSB-resistance related genes in this region. Moreover，bioinformatics analysis and qRT-PCR results provided strong evidence that Csa1G654870 was closely related to GSB resistance process.

Key words: cucumber, introgression line, gummy stem blight, linkage map, BSA-seq, resistance gene