关键词: 葡萄, 伤流液, 内生菌, 鉴定, 抗病, 灰霉病菌

Abstract: In order to explore the roles of grape endophytes played in grey mould resistance，four universal endophyte strains（Rhodotorula mucilaginosa，Cryptococcus，Rahnella aquatilis and Serratia proteamaculans）were closely related，but far from Rhodotorula mucilaginosa and Cryptococcus）were isolated and purified from grape bleeding sap. Morphological observation，physiological characterization and 16S rDNA sequence analysis suggested Rahnella aquatilis and Serratia proteamaculans were closely related，but far from Rhodotorula mucilaginosa and Cryptococcus. In vitro antibacterial effects of the isolated endophytes were tested via plate co-cultivation and grape berry co-infection of both grey mould and the individual endophyte. The results indicated strong inhibition of grey mould’s growth by Cryptococcus，Rahnella aquatilis and Serratia proteamaculans. In addition，berries co-infected with Botrytis cinera and the endophyte showed reduced rate of water loss，increased anti-disease metabolites （flavonoids and total phenols）and the increment activities of SOD，CAT，POD，and PPO，together with the increased expression of disease resistant genes VvPR17，VvDW，VvChi and VvWRKY. These shed lights on the pathways endophytes participating in Botrytis cinera resistance. These results are of practical interest for improving grapevine resistance to grey mould.

Key words: grape, bleeding sap, endophyte, identification, disease resistance, Botrytis cinera