关键词: 柑橘, 柑橘鳞皮病毒, 全基因组, 系统发育分析

Abstract:

Citrus psorosis virus（CPsV）caused a destructive viral disease of citrus，while viral genome information associated with CPsV is still limited to date. In this study，we obtained the complete genome sequences of three CPsV isolates CHN-1，CHN-2 and CHN-3 by using transcriptome sequencing （RNA-Seq），RT-PCR，cloning and Sanger sequencing approaches. Genome sequence analysis showed that the full length sequences of CHN-1，CHN-2 and CHN-3 were 11 282 nucleotides（nt），11 279 nt and 11 278 nt，respectively，and comprised of four open reading frames（ORFs）. Nucleotide sequence similarity between genomic RNAs of the three CPsV isolates ranged from 93.48% to 95.98%，whereas their amino acid（aa）sequence similarities were from 98.18% to 98.86%. Comparing CP genes of the three isolates with six other reported CPsV-isolates，the nucleotide sequence similarities were from 85.83% to 95.23% while amino acid sequence similarities from 94.09% to 99.32%. Phylogenetic analysis indicated that the isolates CHN-1，CHN-2 and CHN-3 clustered together into a branch of evolutionary tree，suggesting they should have the same origin.

Key words: Citrus, Citrus psorosis virus, genome, phylogenetic analysis