关键词: 番茄, 蛋白组学, 红光, 糖代谢

Abstract:

We used tomato（Solanum lycopersicum L.）‘Micro-Tom’as the test material，after 30 days of seed germination. The seedlings were transferred to the artificial climate chamber with red and white（the control）lights. The fruits were harvested at the mature green，breaker and ripe stages，then we determined the fruit soluble sugar content and proteomic. The results showed that the soluble sugar content under red-treatment was higher than the control during the development of tomato fruit，and the difference was significant when compared with the control. The proteomic data showed that，compared with the control，46，134 and 65 differentially abundant proteins were identified in the red-treated tomato fruit，and four，four and seven of them were related to sugar metabolism in the mature green，breaker and ripe stages，respectively. Among the fifteen differentially abundant proteins related to sugar metabolism，nine were up-regulated and six were down-regulated. The differential abundances of the fifteen proteins were validated by determining the expression levels of the corresponding mRNAs by qRT-PCR. With two exceptions of glyceraldehyde-3-phosphate dehydrogenase（GAPN）and acetyl-CoA carboxylase（ACACA），the remaining genes exhibited a similar expression pattern between their mRNA and protein levels，confirming the differential gene expression levels and revealing that transcription levels are not always concomitant to the translation level. Based on the comprehensive analysis，the red light treatment can regulate the sugar content of tomato fruit by regulating some genes and proteins related to sugar metabolism.

Key words: tomato, proteomics, red light, sugar metabolism