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园艺学报 ›› 2018, Vol. 45 ›› Issue (9): 1778-1790.doi: 10.16420/j.issn.0513-353x.2018-0150

• 综述 • 上一篇    下一篇

大蒜分子生物学研究进展

刘宏久1,温艳斌1,刘晓雪1,赵勇强1,黄彩萍2,程智慧1,*   

  1. (1西北农林科技大学园艺学院,陕西杨凌 712100;2西北农林科技大学植物保护学院,陕西杨凌 712100)
  • 出版日期:2018-09-25 发布日期:2018-09-25

A Review for Molecular Biology of Allium sativum

LIU Hongjiu1,WEN Yanbin1,LIU Xiaoxue1,ZHAO Yongqiang1,HUANG Caiping2,and CHENG Zhihui1,*   

  1. (1College of Horticulture,Northwest A & F University,Yangling,Shaanxi 712100,China;2College of Plant Protection,Northwest A & F University,Yangling,Shaanxi 712100,China)
  • Online:2018-09-25 Published:2018-09-25

摘要: 综述了大蒜(Allium sativum L.)分子生物学的研究进展,主要包括5个方面:(1)大蒜遗传多样性分析、遗传图谱构建和资源评价中基因组7种分子标记(RAPD、AFLP、ISSR、SSR、SRAP、InDel和SNP)的开发和利用;(2)大蒜基因组学、表观遗传分析、转录组学和蛋白组学的研究;(3)农杆菌介导法和基因枪法的大蒜遗传转化体系构建;(4)大蒜蒜氨酸酶基因、蒜薹和鳞茎发育基因(gaLFY、AsPI、AsFT1、AsFT2和AsFT4)及抗逆相关基因(AsPCSl、AsMT2a、ASAL和AsNF-YC8)的克隆和表达分析;(5)大蒜分子生物学试验技术(RNA提取、RT-qPCR的内参基因筛选和BAC文库构建)优化。最后指出了当前研究的不足,展望了大蒜分子生物学研究的方向。

关键词: 大蒜, 分子标记, 转录组学, 遗传转化, 蒜氨酸酶

Abstract: This paper reviews the following five aspects in garlic(Allium sativum L.)molecular biology:Firstly,the development and utilization of seven types of garlic genome molecular markers including RAPD,AFLP,ISSR,SSR,SRAP,InDel and SNP in genetic diversity analysis,genetic map construction and germplasm resources evaluation;Secondly,the research of garlic genomics,epigenetic analysis, transcriptomics and proteomics;Thirdly,construction of genetic transformation system including Agrobacterium tumefaciens mediated method and gene gun method;Fourthly,cloning and expression analysis of alliinase gene,genes related to flowering and bulb development(gaLFY,AsPI,AsFT1,AsFT2 and AsFT4)and genes related to stress resistance(AsPCSl,AsMT2a,ASAL and AsNF-YC8);Fifthly,experimental technique optimization of molecular biology of garlic involving RNA extraction,reference gene selection for RT-qPCR and BAC library construction. Finally,the current research defects and prospects of garlic molecular biology are summarized.

Key words: garlic, molecular marker, transcriptomics, genetic transformation, alliinase

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