关键词: 西番莲, 东亚西番莲病毒, 分子鉴定

Abstract: In order to investigate the occurrence of East Asian passiflora virus（EAPV）on Passiflora edulis in Fujian Province，the suspected samples of Passiflora edulis were detected by serological and RT-PCR technology，and the positive samples were further cloned，sequenced and analyzed. The results showed that the serological test of 11 samples were positive for detection of Potyvirus，but all were negative for detection of Soybean mosaic virus（SMV）. The expected fragments of about 660 bp in size were amplified from 11 samples using universal degenerate primers for detection of virus species from the genus Potyvirus. Results of sequence determination and analysis revealed that the sequences of 10 samples were highly consistent with those of the reported Telosma mosaic virus（TeMV）isolates，and the sequence of one sample shared more than 98.3% nucleotide identity with those of the reported EAPV isolates. For the suspected EAPV sample（named FJBXG-P1），the expected size of ≈ 955 fragment was amplified using specific primers. The total length of CP gene of this EAPV-positive sample was 870 nucleotides（GenBank accession number：MG650164）. The sequence of CP gene of FJBXG-P1 were 81.0%–97.6% and 84.0%–96.9% identity with the reported nucleotide sequence and amino acid sequence of EAPV，respectively. Phylogenetic analysis showed that 38 EAPV isolates were clustered into two groups（GroupⅠ and GroupⅡ），in which GroupⅠ was AO strain and Group Ⅱ was IB strain. FJBXG-P1 isolate in this study belonged to AO strain. To the best of our knowledge，EPAV infected Passiflora edulis in Fujian Province is the first report of the virus in mainland China.

Key words: Passiflora edulis, East Asian passiflora virus, molecular identification