关键词: 马铃薯, SSR, 标记开发, 遗传图谱, QTL

Abstract: In the present research，we identified SSRs in the released potato genome sequence by in silico survey and analyzed their distribution. Using developed SSR primers on target chromosomes，the linkage map was condensed and our interested QTLs were reanalyzed. The results showed that there were 28 609 SSR sequences distributed on 12 potato chromosomes. Among them，the numbers of 2 base，3 base and 4 base motif SSR sequences were 15 943，11 053 and 1 613，respectively. The number of repetitive sequences rich in A or T accounted for 88.6%，45.0% and 26.0% of the total number of SSR sequences with 2 base，3 base and 4 base motif，respectively. According to the conserved flanking sequences on both sides of the SSR sequence，40 pairs of primers on chromosome 5（chr05）were designed，of which 35 pairs can be effectively amplified and 20 pairs were shown polymorphic. As a result，32 polymorphic loci of 13 pairs of primers were located on chr05. Utilization of these makers reduces the average marker interval from 9.0 cM to 1.7 cM and the interval of the cold-induced sweetening resistant QTL from 26.0 cM to 4.0 cM. Besides，59 pairs of primers on chromosome 6（chr06）were designed，of which 54 pairs were clearly amplified and 25 pairs shown polymorphic. As a result，30 polymorphic loci of 12 pairs of primers were located on chr06，which reduces the average marker interval from 5.5 cM to 3.5 cM and the interval of the cold-induced sweetening resistant QTL from 9.0 cM to 3.7 cM. The results have laid a good foundation for mapping the genes controlling cold-induced sweetening resistance，and also provided related markers for molecular breeding of potato.

Key words: potato, SSR；marker development, linkage map, QTL