关键词: 柑橘, CsHB1, 启动子, 顺式作用元件, GUS染色

Abstract: Two promoter sequences（1 741 bp and 1 613 bp）of the somatic embryogenesis development-related gene CsHB1 were cloned from genomic DNA of‘Valencia’sweet orange. The sequence similarity of these two promoters is 90.7%. There is a 141 bp fragment difference between them，which contains two Box4 cis-acting elements. The cis-acting elements of promoter were analyzed and predicted using PlantCare databases. The results indicated that the CsHB1 promoter sequence included a variety of response elements，such as endosperm specific elements，meristem expression elements，light-responsive elements，heat stress responsiveness elements，and MYB binding site. In order to study the function of CsHB1 promoter，a promoter-reporter vector prCsHB1::GUS was constructed，and introduced into Arabidopsis by Agrobacterium-mediated method. A total of 13 homozygotes were obtained with prCsHB1-1::GUS，and a total of 10 homozygotes were obtained with prCsHB1-2::GUS. GUS staining activity was detected in different tissues. The results showed that two promoter vectors were transferred and expressed successfully in Arabidopsis，and the GUS activity was mainly detected in callus and anther，not in the pods，blade and seedlings.

Key words: Citrus, CsHB1, promoter, cis-acting element, GUS stainingstaining