缺硼和低pH对枳苗根系细胞壁组分及细胞中硼分布的影响

doi:10.16420/j.issn.0513-353x.2018-0050

摘要/Abstract

摘要： 采用营养液培养的方法，设置pH值为4、5、6，硼浓度为0（缺硼）和10 μmol ? L-1（适宜硼），探究营养液低pH与柑橘砧木枳[Poncirus trifoliate（L）Raf.]幼苗根细胞壁组分及各组分中硼含量的关系。结果表明：①缺硼和低pH值均抑制根系生长；硼适宜时pH 4处理的根系长度比pH 5和pH 6处理减少了26%和29%，但根系干物质量变化不显著；缺硼时pH 4处理的根系干物质量也显著减少。②硼适宜时pH 4处理的地下部硼含量显著增加，且原生质体硼和游离态硼占根硼的比例均降低；缺硼时pH 4处理的原生质体硼占根硼的比例增加，游离态硼占根硼的比例没有显著变化。③缺硼时，pH 4处理细胞壁提取率显著低于pH 6处理。④不论适宜硼或缺硼，pH 4处理均显著降低根系细胞壁中螯合态果胶含量；适宜硼时碱溶性果胶含量pH 4和pH 5处理分别比pH 6减少了26%和18%；缺硼时pH 4处理比pH 5和pH 6处理减少了约20%。⑤硼适宜时根系纤维素含量随pH值降低而降低，pH 4处理比pH 6处理降低了13.8%；缺硼时低pH对根系纤维素含量影响不大。⑥大部分硼都结合在果胶中，且碱溶性果胶中的硼（Alk-B）的比例大于螯合态果胶（Chel-B）中的。以上结果说明，pH值会通过影响根系细胞壁组分及组分中硼含量而影响根系的伸长生长。

关键词: 枳, pH, 细胞壁, 果胶, 纤维素, 硼含量

Abstract: In order to explore changes of cell wall components and boron distribution under low pH in trifoliate root，the different pHs（4，5，6）and boron concentrations（0 and 10 μmol ? L-1）were set up in experiment. The results showed that boron deficiency and low pH could significantly inhibit root growth and elongation. When the boron concentration was suitable，the root length of pH 4 decreased by 26% and 29% compared with that of pH 5 and pH 6. When boron was absent，root dry weight of pH 4 was significantly reduced. However，when adding boron，the dry matter increase of pH 4 was not significant. When boron was enough，the boron content in the underground part of pH 4 increased significantly；compared with pH 6，the proportion of boron in protoplast and free boron to root boron in pH 4 reduced. Compared with pH 6，the proportion of boron in protoplasts to root boron treated by pH 4 increased and the proportion of free boron to root boron was not significantly changed under boron deficiency. Compared with pH 6，the cell wall extraction rate of pH 4 decreased when boron deficiency. Regardless of boron adequate or boron deficiency，pH 4 significantly decreased the content of chelate pectin in the cell wall of the roots of trifoliate. In the suitable boron treatment，the content of alkali soluble pectin treated by pH 4 and pH 5 decreased by 26% and 18% respectively than that of pH 6. The content of alkali soluble pectin treated by pH 4 decreased by about 20% than that of pH 6 and pH 5 treatments under boron deficiency. In addition，when boron deficiency，low pH had little effect on cellulose content in the root of trifoliate. However，the content of cellulose decreased with the decrease of pH value in suitable boron. Compared with pH 6，the cellulose content of pH 4 decreased by 13.8%. Most of the boron was bound to pectin，and the proportion of boron（Alk-B）in alkali soluble pectin was greater than that of chelated pectin（Chel-B）. Obviously，low pH can inhibit the root elongation by affecting the content of boron and components of the root cell wall.

Key words: trifoliate, pH, cell wall, pectin, cellulose, boron content

中图分类号:

S 666

杜晨晴，吴秀文，闫磊，刘亚林，姜存仓*. 缺硼和低pH对枳苗根系细胞壁组分及细胞中硼分布的影响[J]. 园艺学报, 2018, 45(7): 1272-1282.

DU Chenqing，WU Xiuwen，YAN Lei，LIU Yalin，and JIANG Cuncang*. Effect of Boron Deficiency and Low pH on Cell Wall Components and Boron Distribution in Cell of Trifoliate Root[J]. ACTA HORTICULTURAE SINICA, 2018, 45(7): 1272-1282.

[1]	郭丽娜, 王璐, 郝心愿, 祁蒙, 李晓嫚, 王新超, 曾建明. 茶树根系吸收硒的生理特性研究[J]. 园艺学报, 2022, 49(9): 1967-1976.
[2]	刘朝阳, 廖志婵, 路鑫鑫, 何业华. 菠萝类纤维素合成酶CslD家族基因鉴定及AcoCslD2a功能分析[J]. 园艺学报, 2022, 49(8): 1650-1662.
[3]	黄译瑾, 何佳丽, 姜李娜, 曹艳红, 秦嗣军, 吕德国. 果实脆性变化的生理生化研究进展[J]. 园艺学报, 2022, 49(12): 2641-2658.
[4]	李永平, 陈敏氡, 刘建汀, 曾美娟, 朱海生, 温庆放. 黄秋葵纤维素合酶基因家族鉴定及表达分析[J]. 园艺学报, 2022, 49(1): 73-85.
[5]	付茵茵, 闫文潇, 周继磊, 王友晓, 夏阳, 庞彩红, 张士勇, 李双云, . 国槐新品种‘姹紫1号’[J]. 园艺学报, 2021, 48(S2): 3013-3014.
[6]	谢国芳, 刘娜, 宋易, 管春花, 张明生. 菜豆豆荚发育过程中内源激素与细胞壁代谢的关系[J]. 园艺学报, 2021, 48(2): 289-299.
[7]	张晓楠, 余歆, 叶子茂, 刘小丰, 朱延松, 杨胜男, 王旭, 刘梦雨, 赵晓春. 宽皮柑橘果实的剥皮性及与细胞壁多糖的关系[J]. 园艺学报, 2021, 48(12): 2336-2348.
[8]	高萌，屈魏，冉昪，饶景萍*. ‘徐香’与‘海沃德’猕猴桃冷藏期间组织结构与生理变化差异[J]. 园艺学报, 2020, 47(7): 1289-1300.
[9]	翟宇慧，吕嘉琪，李想，罗小宁，李龙，史倩倩*. 欧洲报春细胞液 pH 对花色形成的作用机理初探[J]. 园艺学报, 2020, 47(3): 477-491.
[10]	祝建, 刘本勇, 李述举, 白芝兰, 刘聪, 邓晓东, 张豫, 谢宗周, 刘继红, . 2,4-D在柑橘果实留树保鲜中的作用和可能机制研究[J]. 园艺学报, 2020, 47(11): 2086-2094.
[11]	郭红彦1，白晋华1，段风琴1，郗鑫1，李涛1，郭晋平2,*. 钙处理对‘壶瓶枣’裂果细胞壁降解酶活性及组织结构的影响[J]. 园艺学报, 2019, 46(8): 1486-1494.
[12]	胡健，安玉艳，蔡长玉，何莎莎，汪良驹*. 胞质pH参与5–氨基乙酰丙酸诱导的苹果叶片气孔开放[J]. 园艺学报, 2019, 46(10): 1869-1881.
[13]	刘凌云1,，黄在兴1,2,，邢世和3，翁伯琦4，罗旭辉4，刘朋虎1,**. 灵芝生长过程中培养料中的碳转化及CO2排放[J]. 园艺学报, 2019, 46(10): 2047-2054.
[14]	李艳冰，朱美玉，杨晓茹，李悦，赫易，齐明芳，李天来. 植物中Gibberellin 2-oxidases基因研究进展[J]. 园艺学报, 2018, 45(9): 1844-1856.
[15]	王君秀，袁高鹏，阮科，彭良志，凌丽俐，付行政，淳长品*. 不同pH营养液处理下3种柑橘砧木有机酸分泌的差异[J]. 园艺学报, 2018, 45(6): 1054-1066.