关键词: 菊花, CmDREBa-2, 基因克隆, 生物信息学分析, 表达分析

Abstract: Based on the information of transcriptome database derived from‘C029’，a Puccinia horiana Henn. immune Chrysanthemum morifolium cultivar，the cDNA sequence of DREB（Dehydration responsive element binding protein）was cloned. This DREB gene was named CmDREBa-2. Sequence analysis showed that the open reading frame（ORF）of CmDREBa-2 was 291 bp，encoding 96 amino acids. The molecular weight of the predicted protein was 11 159.04 and the pI value was 11.34. The protein was located in the nucleus，and it was a hydrophilic and unstable protein. The transcription factor of CmDREBa-2 contained the AP2 DNA binding domain which was composed by 49 amino acids. The results of phylogenetic analysis showed that the CmDREBa-2 belonged to the DREB family of A-1 and it had a close relationship with CmDREBa and CmDREBb from C. morifolium. The expression level of CmDREBa-2 reached a maximum at 6 h after Puccinia horiana Henn. infection，with 34 times as much as the control. We also found that the expression of CmDREBa-2 was induced by SA，MeJA and ETH.

Key words: chrysanthemum, CmDREBa-2, cloning, bioinformatics analysis, expression analysis