关键词: 香椿, 遗传多样性, SRAP, EST-SSR

Abstract:

Expressed sequence tags（EST）- simple sequence repeats（SSR）and sequence related amplified polymorphism（SRAP）markers were applied to assess the genetic diversity and genetic relationship of 10 Toona sinensis populations collected from nine provinces in China. A total of 167 polymorphic loci were detected by 33 SRAP primers with 5.06 polymorphic loci per primer，and 221 polymorphic loci were observed by 45 EST-SSR primers with 4.9 alleles per primer. The results of observed number of alleles（EST-SSR，Na = 2.3506），expected heterozygosity（EST-SSR，He = 0.4632）and the Shannon’s information index（SRAP，I = 0.6140；EST-SSR，I = 0.6752）demonstrated that relatively higher level of genetic diversity possessed in these populations of T. sinensis. The genetic differentiation coefficients data（SRAP，Gst = 0.3124；EST-SSR，Fst = 0.2462）also revealed relatively higher level of genetic polymorphisms among these T. sinensis resources. Cluster analysis divided 10 populations into 3 groups，one group consisted of Hengshui，Tai’an and Taihe populations，another group consisted of Chengdu，Kunming，Wuhan and Ruyang populations，Dezhou and Quanzhou populations were belonging to the third group. The results of Nanjing population were different in two kind of markers. Genetic relationship among these T. sinensis populations have no significant correlation with their geographical distribution.

Key words: Toona sinensis, genetic diversity, SRAP, EST-SSR