关键词: 黄瓜, 表皮蜡质, CsWIN1, 功能分析

Abstract:

In Arabidopsis，WIN1/SHINE1 gene has the function of regulating wax synthesis and metabolism. In this study，a homologous gene of WIN1/SHINE1 was cloned from cucumber and named CsWIN1. The results of qRT-PCR showed that CsWIN1 expressed at high levels in the leaves and young fruits of cucumber. Subcellular localization analysis suggested that the CsWIN1 protein is located in the nucleus. The CsWIN1 overexpression in the transgenic plants of Arabidopsis thaliana caused a similar phenotype to WIN1/SHINE1 overexpressing lines of Arabidopsis. The expression analysis of CsWIN1 transgenic lines indicated that the expressions of the genes related to wax synthesis have been changed significantly. These results suggested that the function of CsWIN1 is conserved in the regulation of epidermal wax synthesis through regulating the expression of downstream genes related to wax synthesis to affect wax biosynthesis and metabolism.

Key words: cucumber, epicuticular wax, CsWIN1, functional analysis