关键词: 柿, SSR, 雄性种质资源, 遗传多样性, 亲缘关系

Abstract:

For the scarcity of persimmon male resources，the systematical collection and arrangement of the male resources as well as the clarity of genetic diversity of these resources and the genetic relationship among them together with other main cultivars and closely related species are essential for the efficient utilization of male resources. In this study，more than 90 wild male persimmon germplasm resources were found in Hubei，Guangxi，Jiangsu provinces，etc.，during the survey of wild persimmon germplasm resources nationwide. Subsequently，15 SSR primers were used to analyse the genetic diversity of 47 male persimmon resources，2 andromonoecy resources and 11 Chinese monoecious resources. Sixty-seven female resources，38 Japanese resources（including 25 monoecious resources）and 28 closely related species were used as reference out-group. The results indicated that a total of 144 alleles and 316 genotypes were obtained from 15 loci，with a mean value of 9.60 alleles and 21.07 genotypes per locus. ssrDK11/DQ097479 was the most informative locus. The geographical distribution of male persimmon resources indicated that they were common in wild comfortable remote area. The male resources originated in Mulan Mountain，Hubei Province and in Shaanxi Province showed the highest and the lowest genetic diversity，respectively. The 193 resources were divided into 16 and 12 clusters by UPGMA and ME cluster analysis，respectively. Similar general laws were found according to the two cluster analysis results：（1）a genetic isolation existed among wild male resources from different areas；（2）the wild male resources in Mulan Mountain，Hubei Province could be genetically divided into two clusters，one had a near relationship with Jiangxi，Jiangsu，Hunan wild persimmons as well as main astringent cultivars such as‘Huojing’and monoecious astringent cultivars such as‘Yaoxian Wuhuashi’，while the other was closer to Yunjia Mountain male resources and Luotian non-astringent persimmons（including‘Tianbaogai’）；（3）most Japanese resources could be classified into a same cluster exclusively. 

Key words: persimmon, SSR, male germplasm resources, genetic diversity, genetic relationship