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园艺学报 ›› 2017, Vol. 44 ›› Issue (12): 2361-2371.doi: 10.16420/j.issn.0513-353x.2017-0116

• 研究报告 • 上一篇    下一篇

越橘叶片秋季变色期间花青苷和叶绿素的变化特性

李彬彬,杨俊枫,高 源,潘美娜,侯智霞*   

  1. (北京林业大学省部共建森林培育与保护教育部重点实验室,蓝莓研究与开发中心,北京 100083)
  • 出版日期:2017-12-25 发布日期:2017-12-25

Variations of Anthocyanin and Chlorophyll Contents and Composition in ‘Northland’Blueberry Leaf Throughout the Color Changing Process in Autumn

LI Binbin,YANG Junfeng,GAO Yuan,PAN Meina,and HOU Zhixia*   

  1. (Key Laboratory for Silviculture and Conservation Attached to China Ministry of Education,Blueberry Research and Development Center,Beijing Forestry University,Beijing 100083,China)
  • Online:2017-12-25 Published:2017-12-25

摘要: 以4年生‘北陆’越橘为试材,对秋季叶色变化期间花青苷和叶绿素的含量和组分进行了研究。结果表明,随着叶色变化,花青苷总量升高,越到后期增幅越大,叶绿素总量下降,初期降幅较大;全绿叶片中解析出5种花青苷组分,随叶色变化组分增多,全红叶片中解析出11种组分,分别属于飞燕草素、牵牛花素、锦葵色素和矢车菊素;飞燕草素–3–O–阿拉伯糖苷、牵牛花素–3–O–葡萄糖苷和牵牛花素–3–O–半乳糖苷是主要的花青苷组分,共同主导了花青苷总量的变化趋势;飞燕草素和牵牛花素总量随叶色变化增加,但比值不变;叶绿素a与叶绿素b变化趋势一致,但叶绿素a含量较高,变化幅度较大。综合分析认为,花青苷积累量的增加和叶绿素积累量的减少是叶片变色的关键因素;飞燕草素与牵牛花素是主要的两类花青素,其含量和配比可能决定了叶片的红色;‘北陆’叶色变化过程中主要积累3种花青苷组分:飞燕草素–3–O–阿拉伯糖苷、牵牛花素–3–O–半乳糖苷和牵牛花素–3–O–葡萄糖苷,影响了花青苷总量及主要色素的配比,进一步影响了叶片颜色。

关键词: 越橘, 叶色, 花青苷组分, 叶绿素

Abstract: Change characterizations of the contents and compositions of the anthocyanin and the chlorophyll were studied in 4-year-old‘Northland’blueberry as materials during leaf color transformation in autumn. With the change of leaf color,the total amount of anthocyanin increased,and the greater the increase in the later stages. However,the total amount of chlorophyll decreased with larger decline in the initial stage. Five kinds of anthocyanins were detected in the full green leaf. Along with the leaf color transformation,more anthocyanidins were detected. Eleven kinds of anthocyanins,which belong to 4 kinds of cyanidins:delphinidin,petunidin,malvinidin and peonidin,were detected in the red leaves. Among all the anthocyanins,delphinindin-3-O-arabinoside,petunidin-3-O-galactose and petunidin-3-O-glucose were three kinds of main anthocyanins,which co-led the trend of total anthocyanins. Intrestingly,along with the transformation of leaf color,the total amounts of the delphinidin and petunidin increased,while their ratio remained unchanged. The trend of the Chl.a and Chl.b contents were consistent,in which the Chl.a had higher content and larger change range than Chl.b. The comprehensive analysis showed that the increase of anthocyanin accumulation and the decrease of chlorophyll accumulation were the key factors of leaf color transformation. The delphinidin and petunidin were the main ancyanidins,and their contents and ratio might determined the“red”color of leaf. During leaf color transformation,three kinds of anthocyanidins were mainly accumulated:delphinidin-3-O-arabinoside,petunidin-3-O-galacoside and petunidin-3-O- glucoside,affecting the trend of total amount of anthocyanins,the main pigment ratios and the leaf color,furtherly.

Key words: blueberry, leaf color, anthocyanin fraction, chlorophyll

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