关键词: 草莓, 干旱胁迫, 表观性状, 基因表达

Abstract: In order to understand the feasibility of genetic diagnosis for strawberry under drought stress，it is necessary to recognize the growth and development characteristics of strawberry under drought conditions and information for the expression of important genes and their relationships. Fragaria × ananassa‘Ningyu’was used as material to analyze the growth characteristics and 10 genes expression related to auxin synthesis，abscisic acid synthesis，flower and berry development，pigment synthesis under different drought stress. The results showed that strawberry can complete the life cycle from vegetative growth to reproductive growth under different drought conditions；the selected genes are also exercise and completed its regulation function. Comparing with the control plants，the phenology phases of strawberry under mild or moderate drought conditions with 50%–65% of soil water holding capacity were earlier than in control（80% soil water holding capacity），when severe stress that the soil water holding capacity of 35%，the growth and development of strawberry were delayed，with the increasing of drought stress，strawberry plants，crown diameter，leaves，and berries were become significantly smaller than the control，but with deep color；under different drought conditions，the trend of growth-related gene expression in strawberry were similar basically，the time of gene expression was in accordance with the phase of phenology and the growth length of the corresponding plants under all kinds of drought stress. In mild to moderate drought conditions，the expression time of these genes advanced，with a shorter expression period，but gene expression level increased comparing with the control；while in severe drought conditions，the beginning time of gene expression was close to the control，but the expression period elongated，with low level of gene expression. It can be deduced from gene expression that the mechanism of smaller plant，fewer and smaller berry under light and moderate drought stress might not be the same as that under severe drought stress；the shorter gene expression period and lower expression might be one of the reason which causing the smaller crown diameter，smaller leaves and berries or fewer berries.

Key words: strawberry, drought stress, phenotypic trait, gene expression