关键词: 蛹虫草, Simple Sequence Repeats（SSR）, 遗传相似系数（GS）, 聚类分析

Abstract:

The Cordyceps militaris genome was used as template to identify the SSR loci，to design
the SSR primers，and the subsequent repeatable polymorphic bands were utilized for cluster analysis. The
results showed that 30 SSR primers generated 215 highly repeatable polymorphic bands. Based on the
estimation of genetic similarity coefficient（GS）calculated with unweighted pair group method arithmetic
average（UPGMA）clustering method，the variation range of GS values were from 0.527 to 0.995，and 46
C. militaris strains could be divided into 6 groups with a cutoff of the GS value of 0.761. There was a
certain correlation between the genetic relationship and the geographical origin of the strains.
Interestingly，the strains used for commercial cultivation were genetically similar to each other.

Key words: Cordyceps militaris, simple sequence repeats（SSR）, genetic similarity（GS）, cluster analysis