关键词: 番茄, 黄色柱头, 表型, 遗传

Abstract:

We used Ethyl methanesulfonate（EMS）mutagenesis approach and obtained stable
inherited“yellow stigma”mutant （ys）. Mutagen did not significantly change the chlorophyll and carotenoid
contents in the stigma of mutant ys compared with the wild-type. However，contents of p-coumaric acid
and naringeninchalcones were significantly higher in mutant than that of wild-type. We assumed that
accumulation of this yellow pigment plays important role in tomato yellow stigma formation process. The
result also revealed that there was no significant difference in stigma surface structure，stigma receptivity
and pollen viability of both mutant and wild-type. Furthermore，genetic analysis of various generations
showed that in F1 and BC2 populations all the plants have normal stigma color，as segregation ratios of 3∶1
（P > 0.05）and 1∶1（P > 0.05）was observed in F2 and BC1 populations，respectively. The ratio was
consistent with classical Mendelian law of inheritance，indicating that stigma color may be inherited as a
single recessive nuclear gene. The results of this study provide the material and theoretical basis for the
selection，breeding and improvement of new tomato varieties.

Key words: tomato, yellow stigma, phenotype, inheritance