关键词: 柑橘, 柑橘黄脉病毒, 检测, 序列分析, 进化树

Abstract: In this study，181 citrus samples collected from 12 districts and counties in Zhejiang Province were detected by reverse transcription-polymerase chain reaction（RT-PCR）. The results showed that Citrus yellow vein clearing virus（CYVCV）was first detected in Bergamot（Citrus bergamia）and Ehime Kashi 38（C. reticulata）from Zhejiang Province with an incidence of 68.0% and 47.2%，respectively. The complete genome sequences of 4 CYVCV isolates from Zhejiang Province were sequenced and characterized. Comparisons of the whole genome sequences of these 4 CYVCV isolates as well as 11 isolates previously reported from around the world revealed the sequence identity ranged from 96.9%–99.8% at nucleotide level and 97.9%–99.4% at amino acid level，respectively，indicating that there is a very low level of sequence heterogeneity among CYVCV isolates of different geographic origins and hosts.Although CYVCV isolates from Zhejiang Province were clustered in the same clade in the phylogenetic tree，other isolates from China did not show a relationship with the geographic origin of the sampled trees.

Key words: citrus, Citrus yellow vein clearing virus, detection, sequence alignments, phylogenetic tree analysis