关键词: 葡萄, 果粒形状, SLAF, 基因组关联分析

Abstract: Three hundred and four grape germplasms were selected to do the reduced representation sequencing for genome-wide association mapping to detect the genetic loci controlling berry shape. The DNA samples isolated from the grape germplasms were sequenced with a 8.13-fold sequencing depth for each sample，and a total of 466 618 high quality SLAF（specific locus amplified fragment sequencing）labels were acquired，among which 392 374 were polymorphicones. Alignment analysis to the polymorphic SLAF labels identified 4 241 729 SNP，from which 481 192 SNP were obtained with the screening based on a proportion of genotyped efficiency over 0.8 and minor allele frequencies higher than 0.05. In this study，the loci controlling grape berry shape were the first reported，and the devotions of the gene variances for berry shape were higher than 11% except of the SNP rs26137624 based on GLM. Further analysis using GLM and MLM model detected 7 and 3 SNPs，respectively，and all these 10 SNPs located on chromosome 2. Through filtering the associated upstream and downstream areas around each significant SNP，and detecting these related regions for functional genes，10 genes with predicted functional annotations and 7 without named protein product predicted were found.

Key words: grape, berry shape, SLAF, genome-wide association analysis