关键词: 番茄, 灰霉病, 叶霉病, 假单胞菌, 生物防治

Abstract: In this study，a strain，named as WXCDD51，which has strong antagonistic effects on tomato gray mold disease and tomato leaf mold disease，was screened out from the rhizosphere of healthy tomato plants. Based on the morphological，physiological characteristics and 16S rDNA sequence analysis，WXCDD51 was identified as Pseudomonas sp. Mycelial growth assay results showed that the sterile filtrate of WXCDD51 could significantly inhibit mycelial growth of Botrytis cinerea and Fulvia fulva，and the inhibition rates were 94.29% and 94.06% respectively. The pot experiment results showed that WXCDD51 made effective control of tomato gray mold disease and tomato leaf mold disease，the control efficiencies were 66.23% and 69.45%. By dual culture，strain WXCDD51 had different degrees of inhibition on 10 kinds of plant pathogenic fungi and the inhibition rate was about 53.04%–94.59%. The growth-promoting assay showed that WXCDD51 could promote seeds germination and seedling growth of tomatoes. After seeds were treated by the fermentation liquid of WXCDD51（106 cfu ? mL-1），the germination rate of seeds and radicle length increased 80.0% and 62.57% respectively comparing to the control. Under the treatment of the fermentation liquid of WXCDD51（107 cfu ? mL-1），almost all physiological indexes of treated seedlings were significantly higher than that of the control groups. After tomato fruits were soaked in fermented liquid of WXCDD51（107 cfu ? mL-1），tomato fruits decay can be prevented without affecting tomato fruits’ quality.

Key words: tomato, gray mold disease, leaf mold disease, Pseudomonas sp., biological control