关键词: 茄子, 单性结实, 果实发育, 差异表达序列, 实时荧光定量PCR

Abstract: The expression patterns of 96 Unique ESTs from the eggplant parthenocarpic SSH-cDNA liabries were identified in the fruit different development stages of both of parthenocarpic and non-parthenocarpic eggplant lines by qPCR method. The results showed that，31 ESTs were significantly different in the relative expression in the eggplant fruits（ovary）development under the condition of low temperature，of which 17 ESTs were up-regulated and 14 ESTs were down-regulated. The analyses of sequences of ESTs indicated that 5 sequences were related to resistance to low temperature，6 sequences were associated with plant hormone metabolism. Some sequences were related to the synthesis of protein and carbohydrate in plant metabolic processes，and 1 EST may be a new gene. Together，some differentially expressed sequences could be used as the candidate genes of the parthenocarpy and low temperature tolerance of eggplant.

Key words: eggplant, parthenocarpy, development, differentially expressed sequence, qPCR