关键词: 草莓, 自噬, 胁迫, 基因表达

Abstract: Autophagy plays an important role in the process of plant responses to external stresses，but limited work has been done so far on autophagy in strawberry. For this purpose，the strawberry plants of Fragaria × ananassa‘Ningyu’were kept under darkness，high salinity，flooding，high temperature and drought to explore the role and activities of autophagy in different stress responses and the semi- quantitative and quantitative expression of 6 autophagy-related genes FaATG5，FaATG6，FaATG7，FaATG8C，FaATG8F，FaATG12 involved in strawberry leaves. The results showed：under the condition of five different stresses，the number of autophagosomes in strawberry leaves were all changed，reaching more than 4，which reached up to a maximum of 35 during 3 days under salt stress，the number of autophagosomes increased initially，afterwards decreased under high temperature and drought stress，indicating that both caused more injury to strawberry leaves. The expression of autophagy-related genes showed an increasing trend under the condition of five different stress environments，under high temperature，the expression levels of FaATG5，FaATG7，FaATG8F and FaATG12 increased firstly and then decreased. Similarly，under drought stress condition，the expression levels of FaATG6，FaATG8C and FaATG8F showed high expression level initially then decreased. FaATG8C and FaATG8F are more important in the formation of autophagosome and response to different stresses in strawberry leaves.

Key words: Fragaria × ananassa, strawberry, autophagy, stress, gene expression