关键词: 柑橘, 柑橘黄化脉明病毒, 生物信息学分析, 三基因连锁结构, 亚细胞定位

Abstract: Citrus yellow vein cleansing virus（CYVCV）is a simple positive-sense RNA virus. Its genome contains six open reading frames（ORFs），among which ORF2，ORF3 and ORF4 constitute a triple gene block（TGB）. The TGB gene of CYVCV was amplified and cloned by using the total RNA of infected Eureka lemon[Citrus limon（L.）Burm. F.]. Bioinformatic analysis of TGB encoded proteins were carried out with the physicochemical property and molecular characteristics. The TGB gene subcellular localization vector were constructed through the In-Fusion? technology and transformated to onion epidermal cells mediated by Agrobacterium tumefaciens. Subcellular localization was then observed under fluorescence microscope. Bioinformatics analysis results showed that the physicochemical properties and molecular characteristics of CYVCV-TGB were similar to that of the Potexvirus，and the TGB may involved in virus movement in the host. Subcellular localization results showed that TGBp1 was located on the cell wall（cell membrane），while TGBp2 and TGBp3 were mainly located on the cell wall（cell membrane），with a small amount located in the cell. These results laid the foundation of revealing the CYVCV movement mechanism.

Key words: citrus, Citrus yellow vein clearing virus, bioinformatics analysis, triple gene block；subcellular localization