关键词: 葡萄, CIPK, 基因表达, 非生物逆境

Abstract: Two CIPK genes，VvCIPK13 and VvCIPK14，were isolated from‘Carinena’grapevine（Vitis vinifera）. The full-length of VvCIPK13 was 1 501 bp containing an open reading frame（1 395 bp），which encoded 465 amino acids；the full-length of VvCIPK14 was 1 616 bp containing an open reading frame（1 404 bp），which encoded 468 amino acids. They had the typical kinase domain，NAF regulatory domain and the junction domain，but their similarity was only 40.33%. Real-time PCR analysis indicated that the expressions of VvCIPK13 and VvCIPK14 were higher in tendril than other tissues. Mean- while，VvCIPK13 was induced only by 6-BA，different from that VvCIPK14 was induced by 6-BA，ABA，IAA，SA，MeJA and GA3. The expressions of VvCIPK13 and VvCIPK14 were both induced by salt and drought stresses，but weren’t induced by cold. And the expression of VvCIPK14 was stronger than VvCIPK13. These results showed that VvCIPK13 and VvCIPK14 may play a key role in abiotic stress in grapevine.

Key words: Vitis vinifera, CIPK, gene expression, abiotic stress