关键词: 桃, PpSnRK1β1, PpSnRK1β2, 超表达, 番茄, 光合作用, 碳代谢

Abstract: PpSnRK1 kinase β subunit encoding gene（PpSnRK1β1 and PpSnRK1β2）were primarily cloned from peach [Prunus persica（Linn.）Batsch.] leaves. The CDS of PpSnRK1β1 and PpSnRK1β2 are 720 bp and 867 bp respectively. Real-time quantitative polymerase chain reaction（qRT-PCR）analysis revealed that PpSnRK1β1 expressed the highest in leaves of‘Luxing’peach，while PpSnRK1β2 expressed the highest in it’s fruits，both expressed the lowest in it’s flowers. By Agrobacterium mediated transformation，over-expression of transgenic PpSnRK1β1（line T21 and T23）and PpSnRK1β2（line T91 and T92）tomato plants were obtained. Compared with wild type tomato（WT）activities of SnRK1 in young leaves of line T23 and T91 were increased by 9.84% and 53.32%，respectively. The photosynthetic rate in leaves of T91 was 17.02% higher than that of WT. The soluble sugar content in leaves was increased by 34.00% compared with WT. The starch content in leaves of T91 and T92 was nearly doubled compared with that in WT. Instead，the content of vitamin C in fruits of T23，T91 and T92 decreased by 19.21%，38.49% and 39.76% compared with WT. These data demonstrate that PpSnRK1β1 and PpSnRK1β2 are involved in photosynthesis，and are regulated in carbohydrate metabolism and secondary metabolism.

Key words: Prunus persica, PpSnRK1β1, PpSnRK1β2, over-expression, tomato, photosynthesis, carbohydrate metabolism