关键词: 香樟, CcCBFb, 烟草, 非生物胁迫

Abstract:

In order to explore the function of CcCBFb gene from Cinnamomum camphora，the effect
of over-expression of this gene on abiotic stress resistance improvement is assessed by using transgenic
tobacco. With the identification of positive transgenic tobacco by PCR and semi-quantitative RT-PCR
technique，T1 clonal transgenic tobacco（Line 2 and Line 4）and wild type（WT）would be treated under
the drought，salinity，low temperature and freezing stress. The results showed that survival rate of
transgenic tobacco（Line 2 and Line 4）seedling was better to wild tobacco under drought，salinity stress.
The proline and soluble sugar content of transgenic tobacco plants（Line 2 and Line 4）was higher than
wild tobacco but the content of MDA was lower than wild tobacco under low temperature（4 ℃）treatment.
WT and Line 4 had the different degree wilting but the Line 2 had a good growth status under the freezing
treatment （–4 ℃）with 6 h. So，the overexpression of CcCBFb from camphor tree conferred tobacco plants enhanced the tolerance to cold，drought and salinity stress.

Key words: Cinnamomum camphora, CcCBFb, tobacco, abiotic stress