关键词: 芜菁（蔓菁）, PIP1, 基因克隆, 非生物胁迫, 表达分析

Abstract:

In this study，we cloned the plasma membrane intrinsic proteins1（PIP1）gene from
Brassica rapa‘Tsuda’，which was designated as BrPIP1 with the accession number of KJ173685 in the
NCBI GenBank. The full cDNA sequence of the BrPIP1 gene has 1 056 base pairs，containing an open
reading frame of 861 bp encoding a protein of 286 amino acids. We determined the expression of the
BrPIP1 gene in different tissues and under different abiotic stress conditions ， such as extreme
temperature，dehydration，adverse osmosis，abscisic acid（ABA）and salt stress by quantitative-PCR
analysis. The results demonstrated that the highest expression levels of the BrPIP1 gene could be reached
in the petal，followed by the bud，with tissue specificity. The expression of the BrPIP1 gene was
upregulated under the aforementioned stress conditions，suggesting that the BrPIP1 gene may play a role
in response of abiotic stresses.

Key words: Brassica rapa, PIP1, gene cloning, abiotic stress, gene expression