甜瓜黄斑病毒（MYSV）RT-LAMP快速检测方法的建立

doi:10.16420/j.issn.0513-353x.2016-0923

园艺学报 ›› 2017, Vol. 44 ›› Issue (5): 1005-1010.doi: 10.16420/j.issn.0513-353x.2016-0923

甜瓜黄斑病毒（MYSV）RT-LAMP快速检测方法的建立

李战彪1，谢慧婷1，崔丽贤1，苏琴3，秦碧霞1,*，杨世安1,2，邓铁军1，蔡健和1,*

1广西壮族自治区农业科学院植物保护研究所，广西作物病虫害生物学重点实验室，南宁 530007；2广西大学农学院，南宁 530004；3广西壮族自治区农业科学院微生物研究所，南宁 530007

出版日期:2017-05-25 发布日期:2017-05-25

Establishment of Reverse-transcription Loop-mediated Isothermal Amplication Assay for Rapid Detection of Melon yellow spot virus

LI Zhanbiao1，XIE Huiting1，CUI Lixian1，SU Qin3，QIN Bixia1,*，YANG Shian1,2，DENG Tiejun1，and CAI Jianhe1,*

1Plant Protection Research Institute，Guangxi Academy of Agricultural Sciences，Guangxi Key Laboratory of Biology for Crop Diseases and Insect Pests，Nanning 530007，China；2College of Agriculture，Guangxi University，Nanning 530004，China；3Microbiology Research Institute，Guangxi Academy of Agricultural Sciences，Nanning 530007，China

Online:2017-05-25 Published:2017-05-25

摘要/Abstract

摘要：

根据甜瓜黄斑病毒（Melon yellow spot virus，MYSV）N基因的保守序列设计3组特异引物对，通过一系列优化，筛选并获得1组特异引物，建立了MYSV的RT-LAMP检测方法。该方法可在62 ~ 64 ℃，45 min内完成对样品的检测，并且能够特异性扩增MYSV，灵敏度为RT-PCR的100倍，特异性强，灵敏度高，适合对MYSV病样快速检测与鉴定。

关键词: 甜瓜黄斑病毒, RT-LAMP, 快速检测

Abstract:

Melon yellow spot virus disease caused by（MYSV）Melon yellow spot virus was an newly reported viral disease harm greenhouse melon production in recent years. In this study，three sets of specific primers were designed based on the conserved region of the N gene of MYSV，The reaction system was screened，optimized，and one feasible set of primers was selected to suitable for the RT-LAMP reaction. MYSV RTLAMP optimal reaction parameters were obtained in this study. The established RT-LAMP method was specific in detecting MYSV and can be accomplished in 45 min with highly advantage in sensitivity，100 times than RT-PCR. The method is suitable for rapid and accurate detection and identification of MYSV.

Key words: Melon yellow spot virus（MYSV）, RT-LAMP, rapid detection

李战彪1，谢慧婷1，崔丽贤1，苏琴3，秦碧霞1,*，杨世安1,2，邓铁军1，蔡健和1,*. 甜瓜黄斑病毒（MYSV）RT-LAMP快速检测方法的建立[J]. 园艺学报, 2017, 44(5): 1005-1010.

LI Zhanbiao1，XIE Huiting1，CUI Lixian1，SU Qin3，QIN Bixia1,*，YANG Shian1,2，DENG Tiejun1，and CAI Jianhe1,*. Establishment of Reverse-transcription Loop-mediated Isothermal Amplication Assay for Rapid Detection of Melon yellow spot virus[J]. ACTA HORTICULTURAE SINICA, 2017, 44(5): 1005-1010.

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甜瓜黄斑病毒（MYSV）RT-LAMP快速检测方法的建立

Establishment of Reverse-transcription Loop-mediated Isothermal Amplication Assay for Rapid Detection of Melon yellow spot virus

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甜瓜黄斑病毒（MYSV）RT-LAMP快速检测方法的建立

Establishment of Reverse-transcription Loop-mediated Isothermal Amplication Assay for Rapid Detection of Melon yellow spot virus

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