关键词: 苹果, LysM–类受体激酶, 生物信息学分析, 表达

Abstract:

Conserved protein sequence of LysM（PF01476）and Pkinase（PF00069）domain were used to search the LysM-RLKs from apple（Malus × domestica Borkh.）protein database. The physical and chemical characteristics，evolutionary relationships，chromosomal distribution and gene structure members were analyzed for all of the LysM-RLKs. Additionally，expressions of LysM-RLKs were assayed in specific tissue and development process of apple as well as under biotic stresses based on the published RNA-seq data. In total，12 LysM-RLK genes were identified in the apple genome. The size（amino acid，aa），molecular weight and isoelectric point of LysM-RLKs in apple varied from 553 to 1 053，from 62.65 to 119.04 kD and from 5.11 to 7.45，respectively. The genes mainly sublocalized on plasma membrane. According to phylogenetic analysis，LysM-RLK of apple could be divided into 3 sub-groups. Within a sub-group，LysM-RLKs exhibited similar features of exon-intron structure. Gene expression analysis showed that 4 LysM-RLKs were expressed specifically in different tissue. In addition，expressions of 7 LysM-RLKs were significantly changed when Apple Valsa canker and Apple replant disease occurred. The study contributed to further investigate the functions of LysM-RLKs and disease resistance research in apple.

Key words: apple, LysM-RLK, bioinformatic analysis, expression