关键词: 苹果, 矮化砧木, 水平压条, 绞缢, 内源激素, 生根基因

Abstract:

One-year-old apple dwarfing rootstock‘9-3’was used as material to study the effect of constriction on rooting of layering shoots and the content of endogenous hormones and the expression of rooting related genes during the rooting process. The results showed that the rooting time of shoots treated with constriction was earlier than that of the control，and the rooting rate reached 90.63%，significantly higher than that of the control. At callus formation stage，the content of IAA，ABA and ZR，the ratio of IAA/ABA，IAA/GA₃ and IAA/ZR in the treated shoots were significantly higher than those of the control. Meanwhile the expression of ARRO-1，ARF7 and ARF19 in the treated shoots was also significantly higher than that of the control. During the adventitious-root-formation stage，the content of IAA and GA₃ and the expression of ARRO-1 in the treated shoots were significantly higher than those of the control. At the adventitious-root-formation peak stage，the content of IAA and the ratio of IAA/ABA，IAA/GA₃ and IAA/ZR were significantly lower than those of the control，while the content of ZR and the expression of ARRO-1 were significantly higher than those of the control.

Key words: apple, dwarf rootstock, horizontal layering, constriction, endogenous hormone, rooting gene