关键词: 香石竹, 水孔蛋白, 基因克隆, 表达分析

Abstract:

Ten aquaporin genes were cloned from Dianthus caryophyllus‘Master’based on the analysis from D. caryophyllus and D. chinensis transcriptome，7 genes belonged to PIP subfamily and another 3 belonged to TIP subfamily；DcaAQP1，DcaAQP2，DcaAQP3，DcaAQP4，DcaAQP6，DcaAQP8 and DcaAQP10 had advantage expression in sepals，and DcaAQP5 had predominant expression in stem and petals；DcaAQP7 had advantage expression in leaves；while the DcaAQP9 expressed low in all tissues；During the flowers opening and wilting process，the expression levels of DcaAQP1，DcaAQP3 and DcaAQP6 increased from flower bud stage and to the highest at half bloom stage；DcaAQP4，DcaAQP7，DcaAQP9 and DcaAQP10 expressions were the highest at full bloom stage. DcaAQP2 had the highest expression at flower bud stage；DcaAQP5 reached the highest level at the beginning of wilting stage. Indicating multiple AQP genes acted synergistically to maintain the water uptake and nutrients transport during the flowers opening and wilting process.

Key words: carnation, aquaporin, cloning gene, expression analysis