https://www.ahs.ac.cn/images/0513-353X/images/top-banner1.jpg|#|苹果
https://www.ahs.ac.cn/images/0513-353X/images/top-banner2.jpg|#|甘蓝
https://www.ahs.ac.cn/images/0513-353X/images/top-banner3.jpg|#|菊花
https://www.ahs.ac.cn/images/0513-353X/images/top-banner4.jpg|#|灵芝
https://www.ahs.ac.cn/images/0513-353X/images/top-banner5.jpg|#|桃
https://www.ahs.ac.cn/images/0513-353X/images/top-banner6.jpg|#|黄瓜
https://www.ahs.ac.cn/images/0513-353X/images/top-banner7.jpg|#|蝴蝶兰
https://www.ahs.ac.cn/images/0513-353X/images/top-banner8.jpg|#|樱桃
https://www.ahs.ac.cn/images/0513-353X/images/top-banner9.jpg|#|观赏荷花
https://www.ahs.ac.cn/images/0513-353X/images/top-banner10.jpg|#|菊花
https://www.ahs.ac.cn/images/0513-353X/images/top-banner11.jpg|#|月季
https://www.ahs.ac.cn/images/0513-353X/images/top-banner12.jpg|#|菊花

园艺学报 ›› 2017, Vol. 44 ›› Issue (3): 409-421.doi: 10.16420/j.issn.0513-353x.2016-0530

• 研究论文 •    下一篇

苹果生长素运输基因MdABCB19的克隆及其在矮化砧木中的表达分析

宋春晖,张  东,马娟娟,毛江萍,张宝娟,韩明玉*   

  1. 西北农林科技大学园艺学院,国家苹果改良中心杨凌分中心,陕西果业发展协同创新中心,陕西杨凌 712100
  • 出版日期:2017-03-25 发布日期:2017-03-25
  • 基金资助:

     

    国家现代农业产业技术体系建设专项资金项目(CARS-28);国家星火计划项目(2014GA850002);陕西科技创新工程项目

    (2015NY114);陕西省科技统筹创新工程计划项目(2016KTZDNY01-10)

Isolation and Expression Analysis of MdABCB19 Gene and Its Promoter from Dwarfing Apple Rootstock

SONG Chunhui,ZHANG Dong,MA Juanjuan,MAO Jiangping,ZHANG Baojuan,and HAN Mingyu*   

  1. College of HorticultureNorthwest A & F UniversityYangling Subsidiary Center Project of National Apple Improvement CenterCollaborative Innovation of Center Shaanxi Fruit Industry DevelopmentYanglingShaanxi 712100China
  • Online:2017-03-25 Published:2017-03-25

摘要:

以苹果矮化砧木M9和乔化砧木MM106为材料,克隆到一个3 753 bp的核苷酸序列,其编码1 250个氨基酸,含有两个ABC_membrance结构域,两个ABC_tran结构域,与梨、油菜和拟南芥的ABCB19基因高度同源,命名为MdABCB19该序列M9和MM106中存在一个非同义SNP,编码氨基酸由A突变为S,导致M9 α螺旋少了一段。表达分析表明,MdABCB19在砧木M9和MM106、长富2号/M9和长富2号/MM106均差异表达。启动子序列分析发现M9的MdABCB19启动子在起始密码子上游170 bp处有6个碱基(CTCTGT)缺失,导致缺失一个5'UTR Py-rich stretch motif。MM106MdABCB19启动子活性高于M9,受光照调控。推测M9的MdABCB19启动子5'UTR Py-rich stretch motif缺失可能与其MdABCB19低表达有关;而氨基酸突变导致蛋白质三级结构α螺旋结构改变是否影响生长素运输,有待进一步研究。上述研究表明苹果MdABCB19可能通过调控生长素转运参与砧木苗矮化性状的调控。

关键词: 苹果, 生长素, MdABCB19, 砧木, 矮化

Abstract:

Apple dwarfing rootstock M9 and vigorous rootstock MM106 were used as the material to clone a 3 753 bp nucleotide sequence encoding a length of 1 250 amino acids. The fragment named as MdABCB19 because of high homology with apple(XP 008341564.1),pears(XP 009347047.1),rape(XP 009151836.1),and Arabidopsis(AT3G28860.1)protein sequence of ABCB19. MdABCB19 sequence in M9 and MM106 presented of non-synonymous SNP,leading to the amino acid sequence different at the 885th amino acids:M9 serine(Ser),MM106 alanine(Ala). Protein structure analysis found that the amino acid point mutations,leading to a shorter alpha helix in M9. Real time quantitative expression analysis showed that,MdABCB19 were differentially expressed in the rootstock M9 and MM106 MdABCB19 were also cloned and found“CTCTGT”6 bp deletion at about the 170 bp upstream of the start codon of M9 MdABCB19 promoter,resulting in missing a 5'UTR Py-rich stretch motif. Promoter cis element analysis shown MdABCB19 promoter region contained several light response motifs. Promoter GUS staining shown that MM106 MdABCB19 promoter activity was higher than M9. The MdABCB19 promoter wereregulated by light. M9 MdABCB19 pro-actingmoter 5'UTR Py-rich stretch motif deletions may induce in MdABCB19 low expression in M9.

Key words: apple, auxin, MdABCB19, rootstock, dwarfing

中图分类号: