关键词: 苹果, Mal d 1, 表达分析, 转基因, 斑点落叶病菌

Abstract:

The Mal d 1 gene，which might be involved in the Alternaria blotch resistance in apple，was amplified and sequenced using the total RNA of apple leaves as template. Sequence analyses showed that it was 480 bp in length，contained two exons，and was characterized by bet v 1-like domain. The expressions of Mal d 1 were detected，but varied in apple leaves，flowers，fruit peels and pulps based on qRT-PCR analyses. Along with the development of fruits，it expression in fruit peels was increased first and then decreased. In the inoculated leaves with Alternaria alternata，Mal d 1 was significantly upregulated compared to the control counterpart，indicating is potential role in the resistance against Alternaria blotch. Furthermore，overexpression of Mal d 1 in transgenic callus，which was obtained using PRI101-AN as the expression vector by Agrobacterium-mediated transformation method，could greatly enhance the resistance to Alternaria blotch.

Key words: apple, Mal d 1, expression analysis, transgene, Alternaria alternata apple pathotype