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园艺学报 ›› 2017, Vol. 44 ›› Issue (2): 297-306.doi: 10.16420/j.issn.0513-353x.2016-0491

• 研究论文 • 上一篇    下一篇

嫁接辣椒根系分泌物对根腐病和青枯病的影响

段  曦1,孙晨晨1,孙胜楠1,吴帼秀1,王洪涛2,毕焕改1,艾希珍1,*   

  1. 1山东农业大学园艺科学与工程学院,作物生物学国家重点实验室,农业部黄淮地区园艺作物生物学与种质创制重点开放实验室,山东泰安 2710182山东省枣庄市农业示范园,山东枣庄 277300
  • 出版日期:2017-02-25 发布日期:2017-02-25
  • 基金资助:

    山东省农业重大应用技术创新课题(鲁财农指[2016]36号);‘十二五’国家科技支撑计划项目(2014BAD05B03

Effects of Grafted Pepper Root Exudates on Root Rot and Bacterial Wilt

DUAN Xi1,SUN Chenchen1,SUN Shengnan1,WU Guoxiu1,WANG Hongtao2,BI Huangai1,and AI Xizhen1,*   

  1. 1College of Horticulture Science and EngineeringState Key Laboratory of Crop BiologyKey Laboratory of Horticultural Crop Biology and Germplasm Innovation of Agriculture MinistryShandong Agricultural UniversityTai’anShandong 271018China2Zaozhuang Agriculture Demonstration GardenZaozhuangShandong 277300China
  • Online:2017-02-25 Published:2017-02-25

摘要:

采用浸根法收集辣椒根系分泌物,利用气相色谱—质谱技术(GC–MS)分析其化学组成,研究嫁接辣椒根系分泌物对辣椒根腐病和青枯病病原菌的化感作用。结果表明,砧木、嫁接辣椒和自根辣椒根系分泌物的组分存在较大差异,与自根辣椒根系分泌物相比,砧木和嫁接辣椒的根系分泌物能够抑制根腐病原菌腐皮镰孢菌(Fusarium solani)和青枯病原菌假单胞杆菌(Ralstonia solanacearum)的生长。表明嫁接辣椒根系分泌物组分变化是其减轻土传病害的重要机理之一。通过对砧木、嫁接和自根辣椒根系分泌物组分分析,推测砧木和嫁接辣椒根系分泌物中邻苯二甲酸二异辛酯和二苯并呋喃对病原菌生长起抑制作用,经功能鉴定证明,0.2 mL · L-1的邻苯二甲酸二异辛酯和0.1 g · L-1的二苯并呋喃可降低辣椒发病率,产量分别比对照(清水)高31.7%和38.3%。

关键词: 辣椒, 嫁接, 根系分泌物, 根腐病原菌, 青枯病原菌

Abstract:

To value the effect of grafting on Fusarium solani and Ralstonia solanacearum and effect of root exudates on disease incidence and yield,we anlysized the chemical element of pepper root exudates by Gas Chromatography–Mass Spectrometer(GC–MS),the results indicated remarkable differences of root exudates component among the rootstocks,grafted and own-root pepper. Compared to own-root pepper,the root exudates in grafted pepper and stock could inhibit the growth of pathogens like Fusarium solani and Ralstonia solanacearum. This indicates that the variation of root exudates component in grafted plants which prevented germs growth and reproduction,therefore increased the disease resistance. Based on the GC–MS results,it is supposed that the prevention may be related to 1,2-benzenedicarboxylic acid,diisooctyl ester and dibenzofuran. Further clarified the grafting capsicum can reduce the occurrence of soil-borne disease and continuous cropping obstacle. The function identification proves that 1,2-benzenedicarboxylic acid,diisooctyl ester and dibenzofuran do decrease the disease incidence of pepper and increase the yields by 31.7% and 38.3% respectively compared to the control. The optimum concentration of 1,2-benzenedicarboxylic acid,diisooctyl ester and dibenzofuran for the foliar-spraying is 0.2 mL · L-1 and 0.1 g · L-1.

Key words: grafted, pepper, root exudate, Fusarium solani, Ralstonia solanacearum

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