关键词: 月季, 二倍体, F₁群体, 杂种鉴定, SSR标记, 偏分离

Abstract:

A segregating population of 296 F₁ diploid hybrids has developed from a cross between Rosa chinensis‘Old Blush’（OB）as maternal plant and Rosa wichuriana‘Basye’s Thornless’（W）as paternal plant. In this study，twenty-three SSR markers were found polymorphic within two parents among one hundred and forty-six SSR marks. We selected 94 progenies randomly from F₁ population，used twenty-three SSR marks to test the authenticity of hybrids and the genetic relationship. Three homozygous dominant markers（Fv512，Fv609 and 305）were screened from the SSR marker sets，each marker is enough to identify the entire hybrids in our population. In total，all of progenies were identified as real interspecific hybrids，it suggested that the hybrid reliability is very high in our F₁ population. Twenty markers were used to examine the genotype segregation，chi-square test indicated that nine（45%）markers exhibited segregation distortion with a significance levels at 5% and 1%，it suggested that F₁ population have a high segregation distortion rate and the segregation distortion markers should be excluded when genetic linkage map construction. The UPGMA based dendrograms indicated that the genetic variation and diversity of 94 progenies was significant，and these progenies could be classified into 2 groups and 7 subgroups.

Key words: Rosa chinensis, Rosa wichuriana, diploid, F₁ population, hybrids identification, SSR marker, segregation distortion