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园艺学报 ›› 2016, Vol. 43 ›› Issue (12): 2442-2450.doi: 10.16420/j.issn.0513-353x.2016-0281

• 研究报告 • 上一篇    下一篇

葡萄抗炭疽病QTL的初步定位分析

高晓铭1,2,刘崇怀1,张国海2,张 颖1,姜建福1,孙海生1,樊秀彩1,*   

  1. (1中国农业科学院郑州果树研究所,郑州 450009;2河南科技大学林学院,河南洛阳 471003)
  • 出版日期:2016-12-25 发布日期:2016-12-25

Preliminary Analysis of Grape Anthracnose Resistance QTL

GAO Xiao-ming1,2,LIU Chong-huai1,ZHANG Guo-hai2,ZHANG Ying1,JIANG Jian-fu1,SUN Hai-sheng1,and FAN Xiu-cai1,*   

  1. (1Zhengzhou Fruit Research Institute,Chinese Academy of Agricultural Sciences,Zhengzhou 450009,China;2College of Forestry,Henan University of Science and Technology,Luoyang,Henan 471003,China)
  • Online:2016-12-25 Published:2016-12-25

摘要:

以欧亚种葡萄‘里扎马特’为母本,中国野生刺葡萄‘黑珍珠’为父本进行杂交,随机选择92株后代为作图群体,借助SSR和SRAP分子标记构建遗传图谱,采用区间作图法,对葡萄抗炭疽病相关QTL进行检测并分析。在12号连锁群上检测到1个抗炭疽病相关QTL,其可解释表型变异37.07%,贡献率为71.50%。这表明在12号连锁群检测到的QTL是抗葡萄炭疽病的1个主效QTL。

关键词: 葡萄, 葡萄炭疽病, 抗性, 遗传图谱, QTL

Abstract:

Ninety-two F1 individuals from the interspecific cross between‘Rizamat’(Vitis vinifera)and‘Heizhenzhu’(V. davidii)were used to construct a molecular genetic linkage map by using simple sequence repeats(SSR)and sequence-related amplified polymorphism(SRAP). The interval mapping method was used to detect and analyze the grape anthracnose resistance QTL. A QTL was identified on linkage group 12,which explained 37.07% of the total phenotypic variance,and 71.50% contribution rate of the highest point. It showed that this QTL on linkage group 12 was a major QTL,which controlled the resistance to grape anthracnose.

Key words: Vitis, grape anthracnose, resistance, genetic linkage map, QTL

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