苹果不同继代次数的茎尖组培苗同工酶酶谱及RAPD分析

园艺学报 ›› 2006, Vol. 33 ›› Issue (1): 33-37.

苹果不同继代次数的茎尖组培苗同工酶酶谱及RAPD分析

杜国强¹;师校欣¹;张庆良²;马宝昆¹

(¹ 河北农业大学园艺学院, 保定071001; ²河北省农林科学院棉花研究所, 石家庄050051)

收稿日期:2005-05-12 修回日期:2005-09-13 出版日期:2006-02-25 发布日期:2006-02-25

Isozyme Patterns and RAPD Analysis of Apple Plantlets Repea tedly Subcultured in Vitro

Du Guoqiang¹;Shi Xiaoxin¹;Zhang Qingliang²;Ma Baokun¹

(¹College of Horticulture, Agricultural University of Hebei, Baoding 071001, China; ²Cotton Research Institute, Hebei Academyof Agriculture and Forestry Sciences, Shijiazhuang 050051, China)

Received:2005-05-12 Revised:2005-09-13 Online:2006-02-25 Published:2006-02-25

摘要/Abstract

摘要： 分别对不同继代次数(3～90代) ‘富士’、‘金冠’、‘乔纳金’苹果组培苗进行POX、EST、AMY同工酶分析。结果表明, 同一品种不同继代次数处理间均有一致的酶带谱型。利用超薄层等电聚焦电
泳根据POX酶蛋白pI的差异可区分金冠和乔纳金两品种, 同一品种不同继代次数的组培苗之间pI相同,
组培过程未对POX酶蛋白pI产生影响。利用25个随机引物对不同继代次数的苹果组培继代苗进行了
RAPD分析, 其特征带型表现一致, 未发现变异。说明苹果茎尖离体继代培养90次以内具有较高的遗传稳
定性。

关键词: 苹果, 组织培养苗, 遗传变异, 同工酶酶谱, RAPD

Abstract: The genetic stability of apple plantlets repeatedly subcultured in vitro for three to ninety times
was examined by analysis of isozyme patterns of POX, EST and AMY, aswell as RAPD. The results showed
that there were clear difference in POX, EST, and AMY isozyme patterns among varieties, but there was no
detetable difference in the same varietieswith different times of subculturing. The POX isozyme was further analyzed by using IEF electrophoresis. It was easy to distinguish between genotypes ( i.e. ‘Golden Delicious’
vs. ‘Jonagold’) according to the pI of POX isozyme. However, no difference was found in the same varieties
with different times of subculturing. RAPD analysiswith twenty-five 10-mer arbitrary primers revealed identical bands for plantlets with different times of subculturing within avariety. Our results indicated that genetic
stability of apple plantlets wasmaintained even after repeatedly subcultured in vitro for up to ninety times.

Key words: Apple, Tissue culture seedling, Genetic variation, Isozyme patterns, RAPD

杜国强;师校欣;张庆良;马宝昆. 苹果不同继代次数的茎尖组培苗同工酶酶谱及RAPD分析[J]. 园艺学报, 2006, 33(1): 33-37.

Du Guoqiang;Shi Xiaoxin;Zhang Qingliang;Ma Baokun. Isozyme Patterns and RAPD Analysis of Apple Plantlets Repea tedly Subcultured in Vitro[J]. ACTA HORTICULTURAE SINICA, 2006, 33(1): 33-37.

[1]	于婷婷, 李欢, 宁源生, 宋建飞, 彭璐琳, 贾竣淇, 张玮玮, 杨洪强. 苹果GRAS全基因组鉴定及其对生长素的响应分析[J]. 园艺学报, 2023, 50(2): 397-409.
[2]	韩晓蕾, 张彩霞, 刘锴, 杨安, 严家帝, 李武兴, 康立群, 丛佩华. 中熟苹果新品种‘中苹优蕾’[J]. 园艺学报, 2022, 49(S2): 1-2.
[3]	韩晓蕾, 张彩霞, 刘锴, 严家帝, 李武兴, 康立群, 丛佩华. 中熟苹果新品种‘苹优2号’[J]. 园艺学报, 2022, 49(S1): 1-2.
[4]	王强, 丛佩华, 刘肖烽. 晚熟苹果新品种‘华优甜娃’[J]. 园艺学报, 2022, 49(S1): 3-4.
[5]	王强, 丛佩华, 刘肖烽. 中熟苹果新品种‘华优宝蜜’[J]. 园艺学报, 2022, 49(S1): 5-6.
[6]	杨玲, 丛佩华, 王强, 李武兴, 康立群. 中熟鲜食苹果新品种‘华丰’[J]. 园艺学报, 2022, 49(S1): 7-8.
[7]	丁志杰, 包金波, 柔鲜古丽, 朱甜甜, 李雪丽, 苗浩宇, 田新民. 新疆野苹果与‘元帅’‘金冠’的叶绿体基因组比对研究[J]. 园艺学报, 2022, 49(9): 1977-1990.
[8]	高彦龙, 吴玉霞, 张仲兴, 王双成, 张瑞, 张德, 王延秀. 苹果ELO家族基因鉴定及其在低温胁迫下的表达分析[J]. 园艺学报, 2022, 49(8): 1621-1636.
[9]	郑晓东, 袭祥利, 李玉琪, 孙志娟, 马长青, 韩明三, 李少旋, 田义轲, 王彩虹. 油菜素内酯对盐碱胁迫下平邑甜茶幼苗生长的影响及调控机理研究[J]. 园艺学报, 2022, 49(7): 1401-1414.
[10]	夏炎, 黄松, 武雪莉, 刘一琪, 王苗苗, 宋春晖, 白团辉, 宋尚伟, 庞宏光, 焦健, 郑先波. 基于宏病毒组测序技术的苹果病毒病鉴定与分析[J]. 园艺学报, 2022, 49(7): 1415-1428.
[11]	刘照霞, 张鑫, 王璐, 马玉婷, 陈倩, 朱占玲, 葛顺峰, 姜远茂. 肥料穴施位点对苹果细根生长、¹⁵N吸收利用及产量品质的影响[J]. 园艺学报, 2022, 49(7): 1545-1556.
[12]	马维峰, 李艳梅, 马宗桓, 陈佰鸿, 毛娟. 苹果POD家族基因的鉴定与MdPOD15的功能分析[J]. 园艺学报, 2022, 49(6): 1181-1199.
[13]	冯琛, 黄学旺, 李兴亮, 周佳, 李天红. 不同苹果矮化砧穗组合的抗旱性比较研究[J]. 园艺学报, 2022, 49(5): 945-957.
[14]	张晓云, 唐玉薇, 王凯, 张东, 杨伟伟. 苹果冠层枝梢类型对光能截获效率和光合生产力影响的模拟分析[J]. 园艺学报, 2022, 49(4): 709-722.
[15]	于波, 秦嗣军, 吕德国. 适量稳定供锌促进平邑甜茶幼苗生长和氮的吸收利用[J]. 园艺学报, 2022, 49(3): 473-481.