Cu2+ , Zn2+ 和Mn2+ 对冷胁迫下嫁接黄瓜幼苗叶片提取液SOD活性的影响

园艺学报 ›› 2007, Vol. 34 ›› Issue (4): 895-900.

Cu²⁺ , Zn²⁺ 和Mn²⁺ 对冷胁迫下嫁接黄瓜幼苗叶片提取液SOD活性的影响

李　涛;于贤昌

(山东农业大学园艺科学与工程学院, 作物生物学国家重点实验室, 山东泰安271018)

收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2007-08-25 发布日期:2007-08-25

Effect of Cu²⁺ , Zn²⁺+ and Mn²⁺ on SOD Activity of Cucumber Leaves Extraction after Low Temperature Stress

LI Tao;YU Xian-chang

(College of Horticulture Science and Engineering, State Key Laboratory of Crop Biology, Shandong Agricultural University, Tai'an, Shandong 271018, China)

Received:1900-01-01 Revised:1900-01-01 Online:2007-08-25 Published:2007-08-25

摘要/Abstract

摘要： 以自根和嫁接黄瓜幼苗为试材, 研究叶片Mn、Cu、Zn和Fe含量及低温胁迫下SOD活性的
差异, 结果表明: 嫁接黄瓜叶片Mn、Cu和Zn的含量均显著高于自根黄瓜, 而Fe的含量则显著低于自根黄瓜; 低温胁迫第3天, 嫁接黄瓜叶片SOD、Cu /Zn-SOD和Mn-SOD活性均显著高于自根黄瓜, Fe-SOD则显著低于自根黄瓜。为探索嫁接黄瓜叶片高含量Mn、Cu、Zn与SOD活性之间的关系, 向低温胁迫第3天的自根黄瓜叶片SOD 提取液中加入Mn²⁺ 、Cu²⁺和Zn²⁺ , 使之与嫁接黄瓜叶片SOD 提取液对应组合的Mn²⁺ 、Cu²⁺和Zn²⁺浓度相等, 研究此时的SOD及其同工酶活性的变化, 结果发现: Mn²⁺ 、Cu²⁺和Zn²⁺加入自根黄瓜叶片提取液后均能显著增强SOD活性, 其增强作用的大小依次为Mn²⁺ +Zn²⁺, Mn²⁺ + Cu²⁺,
Mn²⁺, Cu²⁺, Cu²⁺ + Zn²⁺ +Mn²⁺, Cu²⁺ + Zn²⁺和Zn²⁺ 。3种离子的加入都不影响Fe-SOD的活性。这一结果说明黄瓜叶片Mn、Cu和Zn的含量是影响SOD活性大小的重要因素, 嫁接黄瓜吸收Mn、Cu和Zn的能力较强是其SOD活性显著高于自根黄瓜的重要原因之一。

关键词: 黄瓜, 嫁接, 微量元素, 低温胁迫, SOD活性

Abstract: The contents ofMn, Zn, Cu, Fe and the activities of superoxide dismutase ( SOD) and its
isoenzymes in grafted and own-rooted cucumber leaves under low temperature stresswere studied. The results
indicated that the contents ofMn, Cu, Zn and the activities of SOD, Cu /Zn-SOD andMn-SOD in grafted cucumber leaveswere significantly higher than those in own-rooted cucumber leaves, while the content of Fe and
Fe2SOD activity was lower. In order to make clear the relationship s between high contents ofMn, Zn, Cu and SOD activity in grafted cucumber, Mn²⁺ , Zn²⁺ and Cu²⁺ and different combinations were added into own-rooted cucumber leaves extraction, respectively, in the third day during low temperature stress and made their final concentrations were the same as they in grafted cumber leaves extraction. The results showed that adding Mn²⁺ , Zn²⁺ , Cu²⁺ and their different combinations were added into own-rooted cucumber leaves extraction could enhance the SOD and its isoenzymes activities excep t the Fe-SOD. The sequence of increasing level was Mn²⁺ + Zn²⁺ , Mn²⁺ +Cu²⁺, Mn²⁺ , Cu²⁺, Cu²⁺ + Zn²⁺ +Mn²⁺ , Cu²⁺ + Zn²⁺ and Zn²⁺. This re-search has p roved that theMn, Cu and Zn in cucumber leave could affect the SOD activity and the higher SODactivity in grafted cucumber leave might be attributed to higher accumulation of Cu, Zn and Mn.

Key words: Cucumber, Graft, Microelement, Low temperature stress, SOD activity

李　涛;于贤昌. Cu²⁺ , Zn²⁺ 和Mn²⁺ 对冷胁迫下嫁接黄瓜幼苗叶片提取液SOD活性的影响[J]. 园艺学报, 2007, 34(4): 895-900.

LI Tao;YU Xian-chang. Effect of Cu²⁺ , Zn²⁺+ and Mn²⁺ on SOD Activity of Cucumber Leaves Extraction after Low Temperature Stress[J]. ACTA HORTICULTURAE SINICA, 2007, 34(4): 895-900.

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Cu²⁺ , Zn²⁺ 和Mn²⁺ 对冷胁迫下嫁接黄瓜幼苗叶片提取液SOD活性的影响

Effect of Cu²⁺ , Zn²⁺+ and Mn²⁺ on SOD Activity of Cucumber Leaves Extraction after Low Temperature Stress

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