关键词: 中国樱桃, 转录组, SSR, 种质资源, 遗传多样性

Abstract:

Simple sequence repeat（SSR）loci were searched and analyzed transcriptomic data of Prunus pseudocerasus dormancy buds. A total of 7 197 SSR loci were detected which accounted for 15.62% of total unigenes. Dinuclotide repeat were the most abundant SSR type with a frequency of 58.65%，followed by trinucleotide repeat（34.72%）. The availability and polymorphism of selected 8 primers were checked by using 24 cherry accessions. The results showed that average number of effective alleles was 1.73，ranged from a minimum of 1.09（PpSSR8）to a maximum of 2.92（PpSSR2）. Shannon’s indexes ranged from 0.202 to 1.290，with an average of 0.755. The observed heterozygosity and expected heterozygosity were from 0.083 to 0.917 and from 0.082 to 0.671，respectively. The average value of observed heterozygosity was 0.391，and of expected heterozygosity was 0.384. The maximum and minimum values of observed heterozygosity，expected heterozygosity and Shannon’s index were observed at locus PpSSR2 and locus PpSSR8，respectively. Genetic relationship of 24 cherry accessions based on the polymorphic primers were not fully in accordance with morphological evidences. Nevertheless，the cherry germplasms were clearly clustered into 5 clades，which indicated an abundant genetic diversity of P. pseudocerasus gathered from Zhejiang Province. Two landraces sampled from Longquan were dramatically different with other cherry accessions. They are genetically more closed to P. serrulata and P. schneideriana. These landraces could be used as parental lines in cherry breeding and were helpful to enlarge the genetic background of cherry cultivars.

Key words: Prunus pseudocerasus, transcriptome, SSR, germplasm resources, genetic diversity