ALA处理对遮荫下西瓜幼苗叶绿素荧光参数的影响

园艺学报 ›› 2007, Vol. 34 ›› Issue (4): 901-908.

ALA处理对遮荫下西瓜幼苗叶绿素荧光参数的影响

孙永平;汪良驹

(南京农业大学园艺学院, 南京210095)

收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2007-08-25 发布日期:2007-08-25

Effects of 5-Aminolevulinic Acid ( ALA ) on Chlorophyll FluorescenceDynamics of Watermelon Seedlings under Shade Condition

SUN Yong-ping ; WANG Liang-ju

(College of Horticulture, Nanjing Agricultural University, Nanjing 210095, China)

Received:1900-01-01 Revised:1900-01-01 Online:2007-08-25 Published:2007-08-25

摘要/Abstract

摘要： 用100 mg/L 5 - 氨基乙酰丙酸(5-aminolevulinic acid, ALA) 处理遮荫条件下的西瓜幼苗叶片10 d后, 用PAM22100型便携式叶绿素荧光仪连续测定其叶绿素荧光特性。结果表明, 遮荫显著降低叶片光化学效率, 而ALA处理提高弱光下幼苗叶片PSⅡ实际光化学效率(ΦPSⅡ) 、表观光合电子传递速率( ETR) 、光化学猝灭系数( qP ) 以及光化学反应能量分配率( Pc) 。SOD活性抑制剂二乙基二硫代氨基甲酸钠(DDC) 处理降低ΦPSⅡ、ETR、qP和Pc值等荧光参数, 而ALA处理可以消除DDC的抑制效应, 说明ALA对PSⅡ光化学反应的促进效应与细胞抗氧化酶活性有关。遮荫处理提高叶片初始荧光( Fo) , 降低PSⅡ最大光化学效率( Fv/Fm) , 而遮荫下ALA和(或) DDC处理对这些荧光参数变化没有明显效应。据此推测, ALA处理10 d时对西瓜叶片PSⅡ本身没有直接影响, 它对光化学效率的促进效应可能是通过增强PSⅠ附近SOD等抗氧化酶活性, 促进活性氧代谢来实现的。

关键词: 西瓜, 5 - 氨基乙酰丙酸, 叶绿素荧光, 遮荫, 二乙基二硫代氨基甲酸钠

Abstract: The influences of the foliar app lication with 5-aminolevulinic acid (ALA) on dynamics of chlorophyll fluorescence in watermelon (Citrullus lanatus Thunb. Mansfeld) seedlings under shade condition were studied with PAM-2100 Chlorophyll Fluorometer in the work. The results showed that shading significantly depressed chlorophyll fluorescence parameters, whereasALA imp roved the actual photochemical efficiency of photosystemⅡ (ΦPSⅡ) , the electronic transfer rate (ETR) , the photochemical quenching (qP) and the energy distribution in photochemistry (Pc) absorbed by PSⅡreaction center. The treatmentwith 1% diethyldithiocarbamate (DDC), a chelator of Cu²⁺, which inhibits the activity of Cu-Zn-SOD, decreased all the parameters above,
whereas ALA could inverse the inhibition ofDDC, suggesting that p romotion ofALA on photochemical efficiency of PSⅡmight be related with the activities of antioxidant enzymes. The initial fluorescence (Fo) ofwatermelon leaveswas increased by the shade treatment, and companied with a lower level of the maximum photochemical efficiency (Fv/Fm) , suggesting thatwatermelon is a considerably light-loving crop and low light intensity tends to cause ogenesis of photosynthetic apparatus. ButALA and /orDDC did not show any effect on the fluorescence parameterswhich suggested the p romotion ofALA on photochemical efficiency of PSⅡdid not directly relate with the PSⅡreaction centers in watermelon leaves, but related with acceleration of active oxygen metabolism nearby PSⅠreaction centers by increasing the activities of antioxidant enzymes, such as SOD.

Key words: Watermelon, 5-aminolevulinic acid (ALA), Chlorophyll fluorescence, Shade, Diethyldithiocarbamate (DDC)

孙永平;汪良驹. ALA处理对遮荫下西瓜幼苗叶绿素荧光参数的影响[J]. 园艺学报, 2007, 34(4): 901-908.

SUN Yong-ping;WANG Liang-ju. Effects of 5-Aminolevulinic Acid ( ALA ) on Chlorophyll FluorescenceDynamics of Watermelon Seedlings under Shade Condition[J]. ACTA HORTICULTURAE SINICA, 2007, 34(4): 901-908.

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